Table 1
Comparison of in planta BioID techniques
Khan et al.
(2018 ) [13]
Lin et al.
(2018) [12]
Conlan et al.
(2018) [14]
Zhang et al.
(2019) [16]
a
Mair et al.
(2019) [17]
b
Kim et al.
(2019) [18]
c
BirA used
BirA* R118G
BirAG
BirA* R118G
TurboID
BirA* R118G
BioID2
TurboID;
miniTurbo;
BirA* R118G
TurboID
Organism
Arabidopsis thaliana Oryza sativa L. (rice)
Nicotiana
benthamiana
Nicotiana
benthamiana
Arabidopsis thaliana;
Nicotiana
benthamiana
Arabidopsis
thaliana;
Nicotiana
benthamiana
Plant tissue
Leaves from 4–5 week
old plants grown
under 9 h daily light
Protoplasts
Leaves
Leaves
5 day old seedlings
(A.
thaliana);
Leaves
(N.
benthamiana)
14 day old seedlings
(A.
thaliana);
Leaves
(N.
benthamiana)
In planta
expression
method
Stable homozygous
transgenic/
dexamethasoneinducible promoter
Transient
transformations/
maize ubiquitin
promoter
Transient
transformations/
CaMV 35S
promoter
Transient
transformation/
Arabidopsis
ubiquitin-10
promoter or
CAMV 35S
promoter
Stable transgenics/
ubiquitin or gene
specific promoter
(A.
thaliana);
Transient
transformants/
ubiquitin promoter
(N.
benthamiana)
Stable transgenics/
CaMV 35S
promoter
(A.
thaliana);
Transient
transformants/
CaMV 35S
promoter
(N.
benthamiana)
Biotin
application
Leaf infiltration with
needless syringe
Protoplast incubation
in biotin solution
Leaf infiltration with
needless syringe
Leaf infiltration
Vacuum infiltration of
seedlings
(A.
thaliana)
d
or
leaf discs
(N.
benthamiana),
then incubation for
1 h
Unknown
(continued)
Biotin-Based Proximity Labeling of Protein Complexes in Planta
427