independently analyzed via metabolomics. The analysis of variance from this experiment will allow to estimate the variation
between the replicates of same genotype and variation coming
from genotypes. The optimum result is that most of the variance is due to genetic factors with the rest of the error being
split between within replicates from the same plant or replicates
between plants of the same genotype.
5. Plant sampling (harvesting) is a crucial step in sample preparation for metabolomics, and much care needs to be taken here.
As mentioned above, the total variation in the data set is a
Fig. 3 Functional identification of the candidate associations between gamma-L-glutamyl-L-cysteine and a
locus on chromosome 5 harboring CYDS2 and DES1 (see Note 13). (a) Manhattan plots for the metabolite trait
gamma-L-glutamyl-L-cysteine in the control and stress conditions; (b) gamma-L-glutamyl-L-cysteine exhibits
high negative correlations with both candidate genes (DES1 and CYSD2); (c) isotope labeling experiment
assists annotation of chemical formula; (d) Annotation validation for gamma-L-glutamyl-L-cysteine by comparing MS/MS fragmentation pattern with chemical standard; (e) The biochemical pathway that the candidate
associations are involved in
Metabolomic Analysis of Natural Variation in Arabidopsis
403
between the replicates of same genotype and variation coming
from genotypes. The optimum result is that most of the variance is due to genetic factors with the rest of the error being
split between within replicates from the same plant or replicates
between plants of the same genotype.
5. Plant sampling (harvesting) is a crucial step in sample preparation for metabolomics, and much care needs to be taken here.
As mentioned above, the total variation in the data set is a
Fig. 3 Functional identification of the candidate associations between gamma-L-glutamyl-L-cysteine and a
locus on chromosome 5 harboring CYDS2 and DES1 (see Note 13). (a) Manhattan plots for the metabolite trait
gamma-L-glutamyl-L-cysteine in the control and stress conditions; (b) gamma-L-glutamyl-L-cysteine exhibits
high negative correlations with both candidate genes (DES1 and CYSD2); (c) isotope labeling experiment
assists annotation of chemical formula; (d) Annotation validation for gamma-L-glutamyl-L-cysteine by comparing MS/MS fragmentation pattern with chemical standard; (e) The biochemical pathway that the candidate
associations are involved in
Metabolomic Analysis of Natural Variation in Arabidopsis
403