References
1. Xing S, Wallmeroth N, Berendzen KW, Grefen
C (2016) Techniques for the analysis of protein–protein interactions in vivo. Plant Physiol
171(2):727–758
2. Boute N, Jockers R, Issad T (2002) The use of
resonance energy transfer in high-throughput
screening: BRET versus FRET. Trends Pharmacol Sci 23(8):351–354
3. Morell M, Ventura S, Avile ´s FX (2009) Protein
complementation assays: approaches for the
in vivo analysis of protein interactions. FEBS
Lett 583(11):1684–1691
4. Kodama Y, Hu CD (2010) An improved bimolecular fluorescence complementation assay
with a high signal-to-noise ratio. BioTechniques 49(5):793–805
5. Kerppola TK (2008) Bimolecular fluorescence
complementation (BiFC) analysis as a probe of
protein interactions in living cells. Annu Rev
Biophys 37(1):465–487
6. Miller KE, Kim Y, Huh W-K, Park H-O (2015)
Bimolecular fluorescence complementation
(BiFC) analysis: advances and recent applications for genome-wide interaction studies. J
Mol Biol 427(11):2039–2055
7. Shyu YJ, Liu H, Deng X, Hu CD (2006) Identification of new fluorescent protein fragments
for bimolecular fluorescence complementation
analysis under physiological conditions. BioTechniques 40(1):61–66
8. Horstman A, Tonaco IA, Boutilier K, Immink
RG (2014) A cautionary note on the use of
split-YFP/BiFC in plant protein-protein interaction studies. Int J Mol Sci 15(6):9628–9643
9. Cabantous S, Terwilliger TC, Waldo GS
(2005) Protein tagging and detection with
engineered self-assembling fragments of green
fluorescent
protein.
Nat
Biotechnol
23:102–107
10. Brandizzi F, Fricker M, Hawes C (2002) A
greener world: the revolution in plant bioimaging. Nat Rev Mol Cell Biol 3(7):520–530
11. Min MK, Jang M, Lee M, Lee J, Song K, Lee Y,
Choi KY, Robinson DG, Hwang I (2013)
Recruitment of Arf1-GDP to Golgi by Glo3ptype ArfGAPs is crucial for golgi maintenance
and plant growth. Plant Physiol 161
(2):676–691
12. Fujii Y, Kodama Y (2015) In planta comparative analysis of improved green fluorescent proteins with reference to fluorescence intensity
and bimolecular fluorescence complementation ability. Plant Biotechnol 32(1):81–87
13. Henry E, Torun ˜o TY, Jauneau A, Deslandes L,
Coaker G (2017) Direct and indirect visualization of bacterial effector delivery into diverse
plant cell types during infection. Plant Cell 29
(7):1555–1570
14. Park E, Lee H-Y, Woo J, Choi D, DineshKumar SP (2017) Spatiotemporal monitoring
of Pseudomonas syringae effectors via type III
secretion using split fluorescent protein fragments. Plant Cell 29(7):1571–1584
15. Cabantous S, Nguyen HB, Pedelacq JD,
Koraichi F, Chaudhary A, Ganguly K, Lockard
MA, Favre G, Terwilliger TC, Waldo GS
(2013) A new protein–protein interaction sensor based on tripartite split-GFP association.
Sci Rep 3:2854
16. Finnigan GC, Duvalyan A, Liao EN,
Sargsyan A, Thorner J (2016) Detection of
protein–protein interactions at the septin collar
in Saccharomyces cerevisiae using a tripartite
split-GFP system. Mol Biol Cell 27
(17):2708–2725
17. Koraı ¨chi F, Gence R, Bouchenot C,
Grosjean S, Lajoie-Mazenc I, Favre G, Cabantous S (2018) High-content tripartite splitGFP cell-based assays to screen for modulators
of small GTPase activation. J Cell Sci 131(1):
jcs210419
18. Liu TY, Chou WC, Chen WY, Chu CY, Dai
CY, Wu PY (2018) Detection of membrane
protein–protein interaction in planta based
on dual-intein-coupled tripartite split-GFP
association. Plant J 94(3):426–438
19. Zhang J, Wang M, Tang R, Liu Y, Lei C,
Huang Y, Nie Z, Yao S (2018)
Transpeptidation-mediated assembly of tripartite split green fluorescent protein for label-free
assay of sortase activity. Anal Chem 90
(5):3245–3252
20. Foglieni C, Papin S, Salvade ` A, Afroz T,
Pinton
S,
Pedrioli
G,
Ulrich
G,
Polymenidou M, Paganetti P (2017) Split
GFP technologies to structurally characterize
and quantify functional biomolecular interactions of FTD-related proteins. Sci Rep 7
(1):14013
21. Sergiy VA, Nataliia A (2019) Fluorescence protein complementation in microscopy: applications
beyond
detecting
bi-molecular
interactions. Methods Appl Fluoresc 7
(1):012001
22. Chen J, Lalonde S, Obrdlik P, Noorani
Vatani A, Parsa SA, Vilarino C, Revuelta JL,
Frommer WB, Rhee SY (2012) Uncovering
Tripartite Split-sfGFP Assay
335
1. Xing S, Wallmeroth N, Berendzen KW, Grefen
C (2016) Techniques for the analysis of protein–protein interactions in vivo. Plant Physiol
171(2):727–758
2. Boute N, Jockers R, Issad T (2002) The use of
resonance energy transfer in high-throughput
screening: BRET versus FRET. Trends Pharmacol Sci 23(8):351–354
3. Morell M, Ventura S, Avile ´s FX (2009) Protein
complementation assays: approaches for the
in vivo analysis of protein interactions. FEBS
Lett 583(11):1684–1691
4. Kodama Y, Hu CD (2010) An improved bimolecular fluorescence complementation assay
with a high signal-to-noise ratio. BioTechniques 49(5):793–805
5. Kerppola TK (2008) Bimolecular fluorescence
complementation (BiFC) analysis as a probe of
protein interactions in living cells. Annu Rev
Biophys 37(1):465–487
6. Miller KE, Kim Y, Huh W-K, Park H-O (2015)
Bimolecular fluorescence complementation
(BiFC) analysis: advances and recent applications for genome-wide interaction studies. J
Mol Biol 427(11):2039–2055
7. Shyu YJ, Liu H, Deng X, Hu CD (2006) Identification of new fluorescent protein fragments
for bimolecular fluorescence complementation
analysis under physiological conditions. BioTechniques 40(1):61–66
8. Horstman A, Tonaco IA, Boutilier K, Immink
RG (2014) A cautionary note on the use of
split-YFP/BiFC in plant protein-protein interaction studies. Int J Mol Sci 15(6):9628–9643
9. Cabantous S, Terwilliger TC, Waldo GS
(2005) Protein tagging and detection with
engineered self-assembling fragments of green
fluorescent
protein.
Nat
Biotechnol
23:102–107
10. Brandizzi F, Fricker M, Hawes C (2002) A
greener world: the revolution in plant bioimaging. Nat Rev Mol Cell Biol 3(7):520–530
11. Min MK, Jang M, Lee M, Lee J, Song K, Lee Y,
Choi KY, Robinson DG, Hwang I (2013)
Recruitment of Arf1-GDP to Golgi by Glo3ptype ArfGAPs is crucial for golgi maintenance
and plant growth. Plant Physiol 161
(2):676–691
12. Fujii Y, Kodama Y (2015) In planta comparative analysis of improved green fluorescent proteins with reference to fluorescence intensity
and bimolecular fluorescence complementation ability. Plant Biotechnol 32(1):81–87
13. Henry E, Torun ˜o TY, Jauneau A, Deslandes L,
Coaker G (2017) Direct and indirect visualization of bacterial effector delivery into diverse
plant cell types during infection. Plant Cell 29
(7):1555–1570
14. Park E, Lee H-Y, Woo J, Choi D, DineshKumar SP (2017) Spatiotemporal monitoring
of Pseudomonas syringae effectors via type III
secretion using split fluorescent protein fragments. Plant Cell 29(7):1571–1584
15. Cabantous S, Nguyen HB, Pedelacq JD,
Koraichi F, Chaudhary A, Ganguly K, Lockard
MA, Favre G, Terwilliger TC, Waldo GS
(2013) A new protein–protein interaction sensor based on tripartite split-GFP association.
Sci Rep 3:2854
16. Finnigan GC, Duvalyan A, Liao EN,
Sargsyan A, Thorner J (2016) Detection of
protein–protein interactions at the septin collar
in Saccharomyces cerevisiae using a tripartite
split-GFP system. Mol Biol Cell 27
(17):2708–2725
17. Koraı ¨chi F, Gence R, Bouchenot C,
Grosjean S, Lajoie-Mazenc I, Favre G, Cabantous S (2018) High-content tripartite splitGFP cell-based assays to screen for modulators
of small GTPase activation. J Cell Sci 131(1):
jcs210419
18. Liu TY, Chou WC, Chen WY, Chu CY, Dai
CY, Wu PY (2018) Detection of membrane
protein–protein interaction in planta based
on dual-intein-coupled tripartite split-GFP
association. Plant J 94(3):426–438
19. Zhang J, Wang M, Tang R, Liu Y, Lei C,
Huang Y, Nie Z, Yao S (2018)
Transpeptidation-mediated assembly of tripartite split green fluorescent protein for label-free
assay of sortase activity. Anal Chem 90
(5):3245–3252
20. Foglieni C, Papin S, Salvade ` A, Afroz T,
Pinton
S,
Pedrioli
G,
Ulrich
G,
Polymenidou M, Paganetti P (2017) Split
GFP technologies to structurally characterize
and quantify functional biomolecular interactions of FTD-related proteins. Sci Rep 7
(1):14013
21. Sergiy VA, Nataliia A (2019) Fluorescence protein complementation in microscopy: applications
beyond
detecting
bi-molecular
interactions. Methods Appl Fluoresc 7
(1):012001
22. Chen J, Lalonde S, Obrdlik P, Noorani
Vatani A, Parsa SA, Vilarino C, Revuelta JL,
Frommer WB, Rhee SY (2012) Uncovering
Tripartite Split-sfGFP Assay
335