2.4 Consumables
1. 10 μL, 200 μL, 1 mL, and 5 mL pipette tips.
2. Plastic transfer pipettes.
3. 14-mL sterile polystyrene culture tube.
4. 100-mL Erlenmeyer flasks.
5. PCR tubes.
6. 1.5-mL microcentrifuge tubes.
7. 1 mL plastic cuvettes.
8. Petri dishes.
9. Microscope slides and high precision cover-glass.
10. Kimwipes.
11. Micropore surgical tape.
12. Examination gloves.
13. 1-mL plastic syringe, without needle.
14. Whatman filter papers.
15. Sterile 0.45 μm syringe filters.
16. Parafilm.
17. Cling film.
18. Liquid nitrogen
2.5 Equipment
1. Pipettes (2, 20, 200, 1000, and 5000 μL).
2. Stirring bars.
3. Scissors and forceps.
4. Benchtop Dewar Flasks
5. Water bath.
6. 28
C incubator with shaking device.
7. Vortex.
8. Stirrer/heater combination.
9. Microwave.
10. PCR machine.
11. Centrifuge for 1.5- and 2-mL tubes Æ cooling device.
12. Centrifuge with swinging bucket rotor and adaptors for 15-mL
tubes.
13. Spectrophotometer.
14. Ice machine.
15. Rotating mixer
16. Confocal microscope equipped with appropriate excitation and
emission filters or laser units and CCD camera to image GFP
and chlorophyll autofluorescence.
328
Tzu-Yin Liu
1. 10 μL, 200 μL, 1 mL, and 5 mL pipette tips.
2. Plastic transfer pipettes.
3. 14-mL sterile polystyrene culture tube.
4. 100-mL Erlenmeyer flasks.
5. PCR tubes.
6. 1.5-mL microcentrifuge tubes.
7. 1 mL plastic cuvettes.
8. Petri dishes.
9. Microscope slides and high precision cover-glass.
10. Kimwipes.
11. Micropore surgical tape.
12. Examination gloves.
13. 1-mL plastic syringe, without needle.
14. Whatman filter papers.
15. Sterile 0.45 μm syringe filters.
16. Parafilm.
17. Cling film.
18. Liquid nitrogen
2.5 Equipment
1. Pipettes (2, 20, 200, 1000, and 5000 μL).
2. Stirring bars.
3. Scissors and forceps.
4. Benchtop Dewar Flasks
5. Water bath.
6. 28
C incubator with shaking device.
7. Vortex.
8. Stirrer/heater combination.
9. Microwave.
10. PCR machine.
11. Centrifuge for 1.5- and 2-mL tubes Æ cooling device.
12. Centrifuge with swinging bucket rotor and adaptors for 15-mL
tubes.
13. Spectrophotometer.
14. Ice machine.
15. Rotating mixer
16. Confocal microscope equipped with appropriate excitation and
emission filters or laser units and CCD camera to image GFP
and chlorophyll autofluorescence.
328
Tzu-Yin Liu