Figure 4 illustrates establishment of conditions for cytometric
discrimination between pollen and spores based on FS and SS
signals. The various classes of pollen and spores differ in diameters
(see Subheading 2.4) as measured by light microscopy. For
Fig. 4 Using indestructible biological particles to establish optimal sort conditions for large particles with the
BioRad S3. (a) Forward- and Side-Scatter characterization of lycopodium spore and pecan pollen suspensions
and their mixtures. The spores and pollen can be readily distinguished based on these parameters. Post sort
purity: 99.2% (Lycopodium), 90.0% (Pecan). (b) Flow analysis and sorting of mixtures of ragweed and
mulberry pollen. Post sort purity: 97.3% (Ragweed), 100.0% (mulberry)
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281
discrimination between pollen and spores based on FS and SS
signals. The various classes of pollen and spores differ in diameters
(see Subheading 2.4) as measured by light microscopy. For
Fig. 4 Using indestructible biological particles to establish optimal sort conditions for large particles with the
BioRad S3. (a) Forward- and Side-Scatter characterization of lycopodium spore and pecan pollen suspensions
and their mixtures. The spores and pollen can be readily distinguished based on these parameters. Post sort
purity: 99.2% (Lycopodium), 90.0% (Pecan). (b) Flow analysis and sorting of mixtures of ragweed and
mulberry pollen. Post sort purity: 97.3% (Ragweed), 100.0% (mulberry)
Flow Cytometry and Sorting in Arabidopsis
281