2. Keep the plate in the dark for at least 20 min.
3. Measure at the PAM fluorometer the quantum yield of photosystem II (F v /F m , where F v is the difference between the maximal (F m ) and the basal (F 0 ) fluorescence of chlorophyll).
Figure 3 shows that photosynthetic cell cultures maintained in
a culture medium containing 0.5% sucrose, although growing a bit
less than those maintained in 1% sucrose (Fig. 1l), exhibit the
highest photosynthetic efficiency (see Note 25).
Fig. 3 Phenotype and photosynthetic activity of Arabidopsis cell suspension cultures maintained in different
sucrose concentrations. Representative photographs of the flasks and PAM imaging analyses were carried out
on 6-day-old cell suspension cultures. Bar, 1 cm. PAM data are the means Æ SE of three independent
experiments, each one conducted in triplicate. Bars labeled with a different letter differ significantly (P < 0.05)
by Student’s t test. The sample spots shown under the bars are representative of the F v /F m mean value of the
different cell suspension cultures
Cell Suspension Cultures
179
3. Measure at the PAM fluorometer the quantum yield of photosystem II (F v /F m , where F v is the difference between the maximal (F m ) and the basal (F 0 ) fluorescence of chlorophyll).
Figure 3 shows that photosynthetic cell cultures maintained in
a culture medium containing 0.5% sucrose, although growing a bit
less than those maintained in 1% sucrose (Fig. 1l), exhibit the
highest photosynthetic efficiency (see Note 25).
Fig. 3 Phenotype and photosynthetic activity of Arabidopsis cell suspension cultures maintained in different
sucrose concentrations. Representative photographs of the flasks and PAM imaging analyses were carried out
on 6-day-old cell suspension cultures. Bar, 1 cm. PAM data are the means Æ SE of three independent
experiments, each one conducted in triplicate. Bars labeled with a different letter differ significantly (P < 0.05)
by Student’s t test. The sample spots shown under the bars are representative of the F v /F m mean value of the
different cell suspension cultures
Cell Suspension Cultures
179