7. This OD 600 value is approximate. It has been reported that
Agrobacterium at this OD 600 is at optimal conditions for
transfection.
8. Keep the remaining culture in case more culture will be needed
to increase the Agrobacterium OD 600 .
Fig. 2 Transient expression of fluorescent fusion proteins labeling different subcellular compartments in
Arabidopsis seedlings. Confocal scanning micrography (a) GFP–HDEL labels the ER [7]. (b) ST–YFP labels the
Golgi apparatus [8]. (c) GFP-AGP4 labels the plasma membrane [9]. (d) SP-mCherry-C-ter labels the vacuole
[10]. Scale bars: 10 μm
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Ce ´ sar Bernat-Silvestre et al.
Agrobacterium at this OD 600 is at optimal conditions for
transfection.
8. Keep the remaining culture in case more culture will be needed
to increase the Agrobacterium OD 600 .
Fig. 2 Transient expression of fluorescent fusion proteins labeling different subcellular compartments in
Arabidopsis seedlings. Confocal scanning micrography (a) GFP–HDEL labels the ER [7]. (b) ST–YFP labels the
Golgi apparatus [8]. (c) GFP-AGP4 labels the plasma membrane [9]. (d) SP-mCherry-C-ter labels the vacuole
[10]. Scale bars: 10 μm
152
Ce ´ sar Bernat-Silvestre et al.