7. High humidity in the covered tray may induce adventitious
root development in the grafted junction. Trim adventitious
roots to prevent scions from growing as separate plants. To
avoid high humidity, do not water the grafted plants in the
covered tray unless necessary.
8. To grow Arabidopsis rootstocks for hypocotyl grafting under
SD conditions, we usually grow one seedling in a round pot
5.5 cm in diameter and 5 cm high because extra space is
required to manipulate hypocotyl grafting.
9. The LD-grown Arabidopsis can be used for hypocotyl grafting.
We grow the plants under the same conditions for epicotyl
grafting (see Note 1). For hypocotyl grafting under LD conditions, the leaves of rootstocks do not need to be removed.
However, because the hypocotyl of LD-grown plants is fragile,
handle the plants with extra care to prevent damage.
10. For hypocotyl grafting, the scions are attached to the hypocotyl
of rootstocks, so scions usually are shaded by mature leaves of
rootstocks, which greatly hampers the growth vigor of scions.
To improve the growth vigor of the scions, we retain 3–5
young leaves to increase the size of the scions.
11. For hypocotyl grafting, the graft junction is close to the soil,
and high humidity of the soil may cause adventitious root
growth on the scions.
Acknowledgments
This work was supported by grants from Academia Sinica, Taiwan.
References
1. Melnyk CW, Meyerowitz EM (2015) Plant
grafting. Curr Biol 25:R183–R188
2. Cookson SJ, Ollat N (2013) Grafting with
rootstocks induces extensive transcriptional
re-programming in the shoot apical meristem
of grapevine. BMC Plant Biol 13:147–160
3. Wu R, Wang X, Lin Y, Ma Y, Liu G, Yu X,
Zhong S, Liu B (2013) Inter-species grafting
caused extensive and heritable alterations of
DNA methylation in Solanaceae plants. PLoS
One 8:e61995
4. Melnyk CW, Schuster C, Leyser O, Meyerowitz EM (2015) A developmental framework
for graft formation and vascular reconnection
in
Arabidopsis
thaliana.
Curr
Biol
25:1306–1318
5. Melnyk CW, Gabel A, Hardcastle TJ,
Robinson S, Miyashima S, Grosse I,
Meyerowitz EM (2018) Transcriptome
dynamics at Arabidopsis graft junctions reveal
an intertissue recognition mechanism that activates vascular regeneration. Proc Natl Acad Sci
U S A 115:E2337–E2456
6. Tsukaya H, Naito S, Re ´del GP, Komeda Y
(1993) A new class of mutations in Arabidopsis
thaliana, acaulis1, affecting the development
of both inflorescences and leaves. Development
118:751–764
7. Rhee SY, Somerville CR (1995) Flat-surface
grafting in Arabidopsis thaliana. Plant Mol
Biol Rep 13:118–123
8. Haywood V, Yu T-S, Huang N-C, Lucas WJ
(2005) Phloem long-distance trafficking of
GIBBERELLIC ACID-INSENSITIVE RNA
regulates leaf development. Plant J 42:49–68
118
Nien-Chen Huang and Tien-Shin Yu
root development in the grafted junction. Trim adventitious
roots to prevent scions from growing as separate plants. To
avoid high humidity, do not water the grafted plants in the
covered tray unless necessary.
8. To grow Arabidopsis rootstocks for hypocotyl grafting under
SD conditions, we usually grow one seedling in a round pot
5.5 cm in diameter and 5 cm high because extra space is
required to manipulate hypocotyl grafting.
9. The LD-grown Arabidopsis can be used for hypocotyl grafting.
We grow the plants under the same conditions for epicotyl
grafting (see Note 1). For hypocotyl grafting under LD conditions, the leaves of rootstocks do not need to be removed.
However, because the hypocotyl of LD-grown plants is fragile,
handle the plants with extra care to prevent damage.
10. For hypocotyl grafting, the scions are attached to the hypocotyl
of rootstocks, so scions usually are shaded by mature leaves of
rootstocks, which greatly hampers the growth vigor of scions.
To improve the growth vigor of the scions, we retain 3–5
young leaves to increase the size of the scions.
11. For hypocotyl grafting, the graft junction is close to the soil,
and high humidity of the soil may cause adventitious root
growth on the scions.
Acknowledgments
This work was supported by grants from Academia Sinica, Taiwan.
References
1. Melnyk CW, Meyerowitz EM (2015) Plant
grafting. Curr Biol 25:R183–R188
2. Cookson SJ, Ollat N (2013) Grafting with
rootstocks induces extensive transcriptional
re-programming in the shoot apical meristem
of grapevine. BMC Plant Biol 13:147–160
3. Wu R, Wang X, Lin Y, Ma Y, Liu G, Yu X,
Zhong S, Liu B (2013) Inter-species grafting
caused extensive and heritable alterations of
DNA methylation in Solanaceae plants. PLoS
One 8:e61995
4. Melnyk CW, Schuster C, Leyser O, Meyerowitz EM (2015) A developmental framework
for graft formation and vascular reconnection
in
Arabidopsis
thaliana.
Curr
Biol
25:1306–1318
5. Melnyk CW, Gabel A, Hardcastle TJ,
Robinson S, Miyashima S, Grosse I,
Meyerowitz EM (2018) Transcriptome
dynamics at Arabidopsis graft junctions reveal
an intertissue recognition mechanism that activates vascular regeneration. Proc Natl Acad Sci
U S A 115:E2337–E2456
6. Tsukaya H, Naito S, Re ´del GP, Komeda Y
(1993) A new class of mutations in Arabidopsis
thaliana, acaulis1, affecting the development
of both inflorescences and leaves. Development
118:751–764
7. Rhee SY, Somerville CR (1995) Flat-surface
grafting in Arabidopsis thaliana. Plant Mol
Biol Rep 13:118–123
8. Haywood V, Yu T-S, Huang N-C, Lucas WJ
(2005) Phloem long-distance trafficking of
GIBBERELLIC ACID-INSENSITIVE RNA
regulates leaf development. Plant J 42:49–68
118
Nien-Chen Huang and Tien-Shin Yu