2 Materials
1. Arabidopsis seeds from collections of wild accessions, mapping
populations, introgression lines, or mutants. These materials
can be obtained from Arabidopsis stock centers (ABRC:
https://abrc.osu.edu/; NASC: http://arabidopsis.info/).
2. Equipment to grow simultaneously numerous plants and genotypes in replicated designs (pots, trays, labels, soil).
3. Climate sensors including temperature, humidity, and/or light
dataloggers (e.g., iButtons, Measurement System Ltd., or
HOBO, Onset Computer Corporation Inc.).
4. Equipment to keep clean the field site from leaves and debris
(hoe, wire mesh, and forceps).
5. Software for the analysis of nucleotide diversity and environmental associations (see Table 1) and for general (e.g., SAS,
SPSS, STATISTICA or R packages) or specific (e.g., QTL
mapping software; R scripts for evolutionary test) statistics.
Table 1 shows some software and internet addresses containing
relevant information on these materials.
3 Methods
3.1 Growth of
Arabidopsis in Field
Experiments for
Phenotypic Analyses
In order to phenotype Arabidopsis collections of accessions,
mapping populations, or other derived genetic materials such as
introgression lines or mutants, under natural conditions, plants
must be cultivated with a replicated design in a field site until they
complete their life cycle. Figure 1 shows some examples of sites and
field experiments. To perform this:
1. Design the phenotypic assay and the phenological/temporal
schedule for it (see Notes 1–4).
2. Design the experimental setup (number of genotypes, plants
per pot/replicate, replicates per block and blocks; number of
trays or pots), as well as the sowing and/or planting dates,
according to the phenotypes and data pursued (see Notes 1,
3–10).
3. Prepare the area where pots or trays with plants will be placed
(see Notes 11–14).
4. Prepare the seeds or seedlings of the genetic materials that will
be grown together in the same experiment (see Notes 4, 8–10,
15, 16).
5. The day before sowing, prepare the pots/trays with soil, as well
as the labels for pots and blocks (see Notes 17 and 18).
Analyses of Natural Variation
95
1. Arabidopsis seeds from collections of wild accessions, mapping
populations, introgression lines, or mutants. These materials
can be obtained from Arabidopsis stock centers (ABRC:
https://abrc.osu.edu/; NASC: http://arabidopsis.info/).
2. Equipment to grow simultaneously numerous plants and genotypes in replicated designs (pots, trays, labels, soil).
3. Climate sensors including temperature, humidity, and/or light
dataloggers (e.g., iButtons, Measurement System Ltd., or
HOBO, Onset Computer Corporation Inc.).
4. Equipment to keep clean the field site from leaves and debris
(hoe, wire mesh, and forceps).
5. Software for the analysis of nucleotide diversity and environmental associations (see Table 1) and for general (e.g., SAS,
SPSS, STATISTICA or R packages) or specific (e.g., QTL
mapping software; R scripts for evolutionary test) statistics.
Table 1 shows some software and internet addresses containing
relevant information on these materials.
3 Methods
3.1 Growth of
Arabidopsis in Field
Experiments for
Phenotypic Analyses
In order to phenotype Arabidopsis collections of accessions,
mapping populations, or other derived genetic materials such as
introgression lines or mutants, under natural conditions, plants
must be cultivated with a replicated design in a field site until they
complete their life cycle. Figure 1 shows some examples of sites and
field experiments. To perform this:
1. Design the phenotypic assay and the phenological/temporal
schedule for it (see Notes 1–4).
2. Design the experimental setup (number of genotypes, plants
per pot/replicate, replicates per block and blocks; number of
trays or pots), as well as the sowing and/or planting dates,
according to the phenotypes and data pursued (see Notes 1,
3–10).
3. Prepare the area where pots or trays with plants will be placed
(see Notes 11–14).
4. Prepare the seeds or seedlings of the genetic materials that will
be grown together in the same experiment (see Notes 4, 8–10,
15, 16).
5. The day before sowing, prepare the pots/trays with soil, as well
as the labels for pots and blocks (see Notes 17 and 18).
Analyses of Natural Variation
95