95
Table 4.3 List of selected examples of genetically modified microbial biosensors for detection of environmental pollutants
Pollutants
Gene cassette
Detectable output
Detection range
Frames
References
Phenol
fabA
and f
abR
β- galactosidase
1.6–16 ppm
E. coli
Hillson et al. (2007)
2,4-Dichlorophenoxyacetic
acid
EfdRP
DII lux CDABE
Bioluminescence
0.25 mg/L
Rastonia eutropha
Neufeld et al. (2006)
Alkyl sulphonates
Hao-luxAB
Bioluminescence
–
Nitrosomonas europaea
Toba and Hay (2005)
Toluene analogues
P
m -lux
Bioluminescence
0–5 mM
P. putida
mt-2 KG1206
Brandt et al. (2002)
Atrazine
tac-luc-luxAB-aphII
Bioluminescence
7.39 mg/L
Synechocystis
sp.
PCC6803
Kong et al. (2007)
Propazine
3.51 mg/L
Simazine
1.06 mg/L
Methyl mercury
E. coli
with luc
Bioluminescence
50 ng/L
E. coli
Shao et al. (2002)
Dimethyl mercury
2.34 mg/L
Phenolic compounds
capR- lux CDABE
Bioluminescence
0.1–10 mM
E. coli
Ivask et al. (2001)
Naphthalene
nahG- lux CDABE
Bioluminescence
0.5 mg/L
P. fluorescence
Shin et al. (2005)
Pesticides
E. coli
linA2 and
LinA2
Pulsed
Amperometric
2–45 ppt
E. coli
Heitzer et al. (1994)
As
arsR/luxAB
Bioluminescence
4–7 ng/L
E. coli
DH5
Trang et al. (2005)
Hg
merRT-luxCDABE
Luminescence
0.5 ng/L
E. coli
JM109
Larose et al. (2011)
Cu
luxAB
Bioluminescence
300 μg/L
P. fluorescens
DF57-Cu15 Tom-Petersen et al.
(2001)
Cd
cadR-crtI/cadR-lacZ
Red pigment
1–10 mM
Deinococcus radiodurans
Joe et al. (2012)
Cu and Zn
cusC-gfp/rfp;
zraP-gfp/rfp
Fluorescence
26 μM (Cu);16 μM
(Zn)
E. coli
XL1-Blue
Ravikumar et al.
(2012)
Ni
2+
and Co
2+
cnrYXH-luxCDABE
Bioluminescence
9 μM (Ni
2+
); 0.1 μM
(Co
2+
)
RalstoniaeutrophaAE2515
Tibazarwa et al.
(2001)
As
arslux CDABE
Luminescence
0.74–60.00 μg/L
E. coli
Sharma et al. (2013)
4 Genetically Modified Microbial Biosensor for Detection of Pollutants in Water…
Table 4.3 List of selected examples of genetically modified microbial biosensors for detection of environmental pollutants
Pollutants
Gene cassette
Detectable output
Detection range
Frames
References
Phenol
fabA
and f
abR
β- galactosidase
1.6–16 ppm
E. coli
Hillson et al. (2007)
2,4-Dichlorophenoxyacetic
acid
EfdRP
DII lux CDABE
Bioluminescence
0.25 mg/L
Rastonia eutropha
Neufeld et al. (2006)
Alkyl sulphonates
Hao-luxAB
Bioluminescence
–
Nitrosomonas europaea
Toba and Hay (2005)
Toluene analogues
P
m -lux
Bioluminescence
0–5 mM
P. putida
mt-2 KG1206
Brandt et al. (2002)
Atrazine
tac-luc-luxAB-aphII
Bioluminescence
7.39 mg/L
Synechocystis
sp.
PCC6803
Kong et al. (2007)
Propazine
3.51 mg/L
Simazine
1.06 mg/L
Methyl mercury
E. coli
with luc
Bioluminescence
50 ng/L
E. coli
Shao et al. (2002)
Dimethyl mercury
2.34 mg/L
Phenolic compounds
capR- lux CDABE
Bioluminescence
0.1–10 mM
E. coli
Ivask et al. (2001)
Naphthalene
nahG- lux CDABE
Bioluminescence
0.5 mg/L
P. fluorescence
Shin et al. (2005)
Pesticides
E. coli
linA2 and
LinA2
Pulsed
Amperometric
2–45 ppt
E. coli
Heitzer et al. (1994)
As
arsR/luxAB
Bioluminescence
4–7 ng/L
E. coli
DH5
Trang et al. (2005)
Hg
merRT-luxCDABE
Luminescence
0.5 ng/L
E. coli
JM109
Larose et al. (2011)
Cu
luxAB
Bioluminescence
300 μg/L
P. fluorescens
DF57-Cu15 Tom-Petersen et al.
(2001)
Cd
cadR-crtI/cadR-lacZ
Red pigment
1–10 mM
Deinococcus radiodurans
Joe et al. (2012)
Cu and Zn
cusC-gfp/rfp;
zraP-gfp/rfp
Fluorescence
26 μM (Cu);16 μM
(Zn)
E. coli
XL1-Blue
Ravikumar et al.
(2012)
Ni
2+
and Co
2+
cnrYXH-luxCDABE
Bioluminescence
9 μM (Ni
2+
); 0.1 μM
(Co
2+
)
RalstoniaeutrophaAE2515
Tibazarwa et al.
(2001)
As
arslux CDABE
Luminescence
0.74–60.00 μg/L
E. coli
Sharma et al. (2013)
4 Genetically Modified Microbial Biosensor for Detection of Pollutants in Water…