PART VI SPECIFIC TECHNIQUES FOR THE ANALYSIS OF RNA FUNCTIONS
23 Integrated Genome-Scale Analysis and Northern Blot Detection
of Retrotransposon siRNAs Across Plant Species. . . . . . . . . . . . . . . . . . . . . . . . . . . . 387
Marcel Bo ¨hrer, Bart Rymen, Christophe Himber, Aude Gerbaud,
David Pflieger, Debbie Laudencia-Chingcuanco, Amy Cartwright,
John Vogel, Richard Sibout, and Todd Blevins
24 Transfection of Small Noncoding RNAs into Arabidopsis thaliana
Protoplasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 413
Ste ´phanie Lalande, Marjorie Chery, Elodie Ubrig, Guillaume Hummel,
Julie Kubina, Ange `le Geldreich, and Laurence Drouard
25 In Vivo Reporter System for miRNA-Mediated RNA Silencing . . . . . . . . . . . . . . . 431
Ka ´ roly Fa ´ tyol
26 TRAP-SEQ of Eukaryotic Translatomes Applied to the Detection
of Polysome-Associated Long Noncoding RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . 451
Soledad Traubenik, Flavio Blanco, Marı ´a Eugenia Zanetti,
and Mauricio A. Reynoso
27 Posttranscriptional Suzuki-Miyaura Cross-Coupling Yields Labeled
RNA for Conformational Analysis and Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . 473
Manisha B. Walunj and Seergazhi G. Srivatsan
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 487
Contents
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23 Integrated Genome-Scale Analysis and Northern Blot Detection
of Retrotransposon siRNAs Across Plant Species. . . . . . . . . . . . . . . . . . . . . . . . . . . . 387
Marcel Bo ¨hrer, Bart Rymen, Christophe Himber, Aude Gerbaud,
David Pflieger, Debbie Laudencia-Chingcuanco, Amy Cartwright,
John Vogel, Richard Sibout, and Todd Blevins
24 Transfection of Small Noncoding RNAs into Arabidopsis thaliana
Protoplasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 413
Ste ´phanie Lalande, Marjorie Chery, Elodie Ubrig, Guillaume Hummel,
Julie Kubina, Ange `le Geldreich, and Laurence Drouard
25 In Vivo Reporter System for miRNA-Mediated RNA Silencing . . . . . . . . . . . . . . . 431
Ka ´ roly Fa ´ tyol
26 TRAP-SEQ of Eukaryotic Translatomes Applied to the Detection
of Polysome-Associated Long Noncoding RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . 451
Soledad Traubenik, Flavio Blanco, Marı ´a Eugenia Zanetti,
and Mauricio A. Reynoso
27 Posttranscriptional Suzuki-Miyaura Cross-Coupling Yields Labeled
RNA for Conformational Analysis and Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . 473
Manisha B. Walunj and Seergazhi G. Srivatsan
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 487
Contents
ix