2.4 Enzymes
and Other Reagents
1. Cellulase R10 (Yakult Pharmaceutical Industry Co., Ltd).
2. Macerozyme R10 (Yakult Pharmaceutical Industry Co., Ltd).
3. 10% Acetylated BSA.
4. PEG-4000.
5. RNase A/T1 mix: 2 mg/mL RNase A, 5000 units/mL
RNase T1.
6. 20 mg/mL Glycogen.
7. 10 units/μL Polynucleotide kinase.
8. 10Â Polynucleotide kinase buffer.
9. 10 μCi/μL (2500 Ci/mmole) Radiolabeled [γ
32 P]-adenosine
triphosphate (ATP).
2.5 Equipment
and Miscellaneous
Material
1. Erlenmeyer of 250 mL.
2. Water bath.
3. Centrifuge allowing deceleration without brake, with rotors
compatible for 2 mL, 14 mL, and 50 mL tubes.
4. 2 mL Round-bottom tubes.
5. 14 mL Round-bottom tubes.
6. 50 mL Tubes.
7. Wide-mouth pipettes (tips of at least a 2 mm diameter).
8. Micropipette tips cut at their extremity to enlarge the opening
(2 mm diameter).
9. Incubator.
10. Petri dishes (100 mm diameter; 20 mm height).
11. Square Petri dish (120 mm  120 mm).
12. Scalpel blade.
13. 12-Well plate (3.65 cm
2 /well) for cell culture.
14. Micropore gas-permeable tape.
15. Orbital shaker.
16. Tube rotator.
17. Vacuum chamber.
18. Miracloth.
19. Inverted bright-field microscope.
20. Fuchs-Rosenthal cell.
21. Mini PROTEAN™ TetraHandcast System (Biorad).
22. Mini PROTEAN™ TetraCell System (Biorad).
23. 15-Well comb.
24. Power supply Power Pac basic (Biorad).
25. UV transilluminator.
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and Other Reagents
1. Cellulase R10 (Yakult Pharmaceutical Industry Co., Ltd).
2. Macerozyme R10 (Yakult Pharmaceutical Industry Co., Ltd).
3. 10% Acetylated BSA.
4. PEG-4000.
5. RNase A/T1 mix: 2 mg/mL RNase A, 5000 units/mL
RNase T1.
6. 20 mg/mL Glycogen.
7. 10 units/μL Polynucleotide kinase.
8. 10Â Polynucleotide kinase buffer.
9. 10 μCi/μL (2500 Ci/mmole) Radiolabeled [γ
32 P]-adenosine
triphosphate (ATP).
2.5 Equipment
and Miscellaneous
Material
1. Erlenmeyer of 250 mL.
2. Water bath.
3. Centrifuge allowing deceleration without brake, with rotors
compatible for 2 mL, 14 mL, and 50 mL tubes.
4. 2 mL Round-bottom tubes.
5. 14 mL Round-bottom tubes.
6. 50 mL Tubes.
7. Wide-mouth pipettes (tips of at least a 2 mm diameter).
8. Micropipette tips cut at their extremity to enlarge the opening
(2 mm diameter).
9. Incubator.
10. Petri dishes (100 mm diameter; 20 mm height).
11. Square Petri dish (120 mm  120 mm).
12. Scalpel blade.
13. 12-Well plate (3.65 cm
2 /well) for cell culture.
14. Micropore gas-permeable tape.
15. Orbital shaker.
16. Tube rotator.
17. Vacuum chamber.
18. Miracloth.
19. Inverted bright-field microscope.
20. Fuchs-Rosenthal cell.
21. Mini PROTEAN™ TetraHandcast System (Biorad).
22. Mini PROTEAN™ TetraCell System (Biorad).
23. 15-Well comb.
24. Power supply Power Pac basic (Biorad).
25. UV transilluminator.
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Ste ´ phanie Lalande et al.