References
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6. Bleckmann A, Dresselhaus T (2016) Fluorescent
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(F-WISH) in plant germ cells and the fertilized
ovule. Methods 98:66–73. https://doi.org/10.
1016/j.ymeth.2015.10.019
RNA-FISH
21
1. Le ´cuyer E, Yoshida H, Parthasarathy N et al
(2007) Global analysis of mRNA localization
reveals a prominent role in organizing cellular
architecture and function. Cell 131:174–187.
https://doi.org/10.1016/j.cell.2007.08.003
2. Lawrence JB, Singer RH (1986) Intracellular
localization of messenger RNAs for cytoskeletal
proteins. Cell 45:407–415. https://doi.org/10.
1016/0092-8674(86)90326-0
3. Ghosh Dastidar M, Mosiolek M, Bleckmann A
et al (2016) Sensitive whole mount in situ localization of small RNAs in plants. Plant J
88:694–702.
https://doi.org/10.1111/tpj.
13270
4. Jackson DP (1992) In situ hybridization in
plants. In: Bowles DJ, Gurr SJ, McPherson M
(eds) Molecular plant pathology: a practical
approach series 1. Oxford University Press,
Oxford, pp 163–174
5. Heja ´tko J, Blilou I, Brewer PB et al (2006) In
situ hybridization technique for mRNA detection in whole mount Arabidopsis samples. Nat
Protoc 1:1939–1946. https://doi.org/10.
1038/nprot.2006.333
6. Bleckmann A, Dresselhaus T (2016) Fluorescent
whole-mount RNA in situ hybridization
(F-WISH) in plant germ cells and the fertilized
ovule. Methods 98:66–73. https://doi.org/10.
1016/j.ymeth.2015.10.019
RNA-FISH
21
