References
1. Laliberte ´ J-F, Zheng H (2014) Viral manipulation of plant host membranes. Annu Rev Virol
1:237–259.
https://doi.org/10.1146/
annurev-virology-031413-085532
2. Wang A (2015) Dissecting the molecular network of virus-plant interactions: the complex
roles of host factors. Annu Rev Phytopathol
53:45–66.
https://doi.org/10.1146/
annurev-phyto-080614-120001
3. Nagy PD (2016) Tombusvirus-host interactions: co-opted evolutionarily conserved host
factors take center court. Annu Rev Virol
3:491–515.
https://doi.org/10.1146/
annurev-virology-110615-042312
4. Dufresne PJ, Thivierge K, Cotton S,
Beauchemin C, Ide C, Ubalijoro E, Laliberte ´
J-F, Fortin MG (2008) Heat shock 70 protein
interaction with Turnip mosaic virus
RNA-dependent RNA polymerase within
virus-induced membrane vesicles. Virology
374:217–227.
https://doi.org/10.1016/j.
virol.2007.12.014
5. Lohmus A, Varjosalo M, Makinen K (2016)
Protein composition of 6K2-induced membrane structures formed during Potato virus A
infection. Mol Plant Pathol 17:943–958.
https://doi.org/10.1111/mpp.12341
6. Wang X, Cao X, Liu M, Zhang R, Zhang X,
Gao Z, Zhao X, Xu K, Li D, Zhang Y (2018)
Hsc70-2 is required for Beet black scorch virus
infection through interaction with replication
and capsid proteins. Sci Rep 8:4526. https://
doi.org/10.1038/s41598-018-22778-9
7. Monsion B, Incarbone M, Hleibieh K,
Poignavent V, Ghannam A, Dunoyer P,
Daeffler L, Tilsner J, Ritzenthaler C (2018)
Efficient detection of long dsRNA in vitro
and in vivo using the dsRNA binding domain
from FHV B2 protein. Front Plant Sci 9:70.
https://doi.org/10.3389/fpls.2018.00070
8. Incarbone M, Monsion B, Kuhn L, Scheer H,
Poignavent V, Dunoyer P, Ritzenthaler C
(2019) Immunocapture of dsRNA-bound proteins provides insight into Tobacco rattle virus
replication complexes. bioRxiv. https://doi.
org/10.1101/842666
9. Lampropoulos A, Sutikovic Z, Wenzl C,
Maegele I, Lohmann JU, Forner J (2013)
GreenGate - A novel, versatile, and efficient
cloning system for plant transgenesis. PLoS
One 8:e83043. https://doi.org/10.1371/
journal.pone.0083043
10. Wang D, Maule AJ (1995) Inhibition of host
gene expression associated with plant virus replication. Science 267:229–231. https://doi.
org/10.1126/science.267.5195.229
Pull-Down of Viral Double-Stranded RNA
327
1. Laliberte ´ J-F, Zheng H (2014) Viral manipulation of plant host membranes. Annu Rev Virol
1:237–259.
https://doi.org/10.1146/
annurev-virology-031413-085532
2. Wang A (2015) Dissecting the molecular network of virus-plant interactions: the complex
roles of host factors. Annu Rev Phytopathol
53:45–66.
https://doi.org/10.1146/
annurev-phyto-080614-120001
3. Nagy PD (2016) Tombusvirus-host interactions: co-opted evolutionarily conserved host
factors take center court. Annu Rev Virol
3:491–515.
https://doi.org/10.1146/
annurev-virology-110615-042312
4. Dufresne PJ, Thivierge K, Cotton S,
Beauchemin C, Ide C, Ubalijoro E, Laliberte ´
J-F, Fortin MG (2008) Heat shock 70 protein
interaction with Turnip mosaic virus
RNA-dependent RNA polymerase within
virus-induced membrane vesicles. Virology
374:217–227.
https://doi.org/10.1016/j.
virol.2007.12.014
5. Lohmus A, Varjosalo M, Makinen K (2016)
Protein composition of 6K2-induced membrane structures formed during Potato virus A
infection. Mol Plant Pathol 17:943–958.
https://doi.org/10.1111/mpp.12341
6. Wang X, Cao X, Liu M, Zhang R, Zhang X,
Gao Z, Zhao X, Xu K, Li D, Zhang Y (2018)
Hsc70-2 is required for Beet black scorch virus
infection through interaction with replication
and capsid proteins. Sci Rep 8:4526. https://
doi.org/10.1038/s41598-018-22778-9
7. Monsion B, Incarbone M, Hleibieh K,
Poignavent V, Ghannam A, Dunoyer P,
Daeffler L, Tilsner J, Ritzenthaler C (2018)
Efficient detection of long dsRNA in vitro
and in vivo using the dsRNA binding domain
from FHV B2 protein. Front Plant Sci 9:70.
https://doi.org/10.3389/fpls.2018.00070
8. Incarbone M, Monsion B, Kuhn L, Scheer H,
Poignavent V, Dunoyer P, Ritzenthaler C
(2019) Immunocapture of dsRNA-bound proteins provides insight into Tobacco rattle virus
replication complexes. bioRxiv. https://doi.
org/10.1101/842666
9. Lampropoulos A, Sutikovic Z, Wenzl C,
Maegele I, Lohmann JU, Forner J (2013)
GreenGate - A novel, versatile, and efficient
cloning system for plant transgenesis. PLoS
One 8:e83043. https://doi.org/10.1371/
journal.pone.0083043
10. Wang D, Maule AJ (1995) Inhibition of host
gene expression associated with plant virus replication. Science 267:229–231. https://doi.
org/10.1126/science.267.5195.229
Pull-Down of Viral Double-Stranded RNA
327