mRNA tethered to the N-terminal domain of SKIP (Kinesin 1-binding protein)
Snapshot of the cell
Max-intesity projection
of the selected particle
Snapshot of the cell
Max-intesity projection
of the selected particle
a
b
Average lifetimes
τ 1 : 0.65000 (sec)
τ 2 : 1.20000 (sec)
ML (estimated) parameters
D 1 : 0.00740 (μm
2
S
-1
)
V 1 : [0.00000;0.00000] (μm/s)
D 2 : 0.00740 (μm
2
S
-1
)
V 2 : [0.50701;-1.61018] (μm/s)
Trajectory (ML state sequence)
Trajectory (ML state sequence)
1.0
0.9
0.8
0.7
D
DV
D, D
D, DV
DV, DV
D, D, D
D, D, DV
D, DV, DV
DV, DV, DV
0.5
0.4
0.3
0.2
0.1
0.6
0
Model probability
Δx (μm)
Δy (μm)
-0.05 0 0.05 0.1
0
-0.05
-0.1
-0.15
-0.2
-0.25
-0.1
-0.05
-0.1
Trajectory displacements
0.9
0.8
0.7
D
DV
D, D
D, DV
DV, DV
D, D, D
D, D, DV
D, DV, DV
DV, DV, DV
0.5
0.4
0.3
0.2
0.1
0.6
0
Model probability
Δx (μm)
Δy (μm)
0
0.2
0.2
0.1
0
-0.1
-0.2
-0.3
0.2
0.4
0.5
Trajectory displacements
0.3
ML (estimated) parameters
D: 0.18942 (μm
2 S
-1
)
V: [0.00000;0.00000] (μm/s)
21.4
21.8
22.2
22.6
29.5
30
30.5
22.4
22.0
21.6
28.0
28.4
28.8
29.8
36.5 37 37.5
29.4
28.6
28.2
27.8
29.0
29.2
29.6
36
Fig. 3 Analysis and quantification of mobility of particles undergoing directed motion. (a) A representative
image of a cell that is transfected with the MCP-GFP-FLuc plasmid is shown. (b) A representative image of a
Tethering Assay for RNA Mobility
279