a
c
b
d
mRNA tethered to
firefly luciferase
mRNA tethered to
RILPL2 RH1 domain
0.00
0.05
0.10
0.15
0.20
Fluc
RILPL2−RH1
Diffusion Coefficient of
fast moving population (μm
2
S
-1
)
0.00
0.02
0.04
Fluc
RILPL2−RH1
Diffusion Coefficient of
slow moving population (μm
2
S
-1
)
D = 0.046 μm
2 S
-1
D1 = 0.169 μm
2 S
-1 (32.941%)
D2 = 0.021 μm
2 S
-1 (67.059%)
D = 0.032 μm
2 S
-1
D1 = 0.080 μm
2 S
-1 (57.026%)
D2 = 0.001 μm
2 S
-1 (42.974%)
Displacement (μm)
Displacement (μm)
0.2
0.4
0.6
0.8
Fluc
RILPL2−RH1
Fraction of fast moving population
0.2
0.4
0.6
0.8
Fluc
RILPL2−RH1
Fraction of slow moving population
0.05
0.03
0.01
0.1 0.2 0.3 0.4 0.5 0.6 0.7
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
Cumulated Probability
0.1
0.2
0.3
0.4
0.5
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
Cumulated Probability
Fig. 2 Analysis and quantification of mobility of RNA particles that are undergoing diffusive and/or restricted
motion. (a) Top panel shows a representative image of a cell that is transfected with the MCP-GFP-Firefly
Tethering Assay for RNA Mobility
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