4. Rotator.
5. Incubator at 37
C.
6. Freezer at À80
C.
7. Cold room at 4
C.
8. Mass spectrometer.
Fig. 1 An overview of RNA-affinity pulldown. After oxidation, RNA probes are
covalently tagged with agarose beads. Incubation of the tagged RNA probes and
protein lysates encourages the formation of RNPs. After several washes to
remove nonspecific binding, the RNP complex is pulled down and the proteins
that interact with the RNA sequence of interest are subsequently identified using
mass spectrometry
Using Bead-Tagged RNAs to Identify RBPs
243
5. Incubator at 37
C.
6. Freezer at À80
C.
7. Cold room at 4
C.
8. Mass spectrometer.
Fig. 1 An overview of RNA-affinity pulldown. After oxidation, RNA probes are
covalently tagged with agarose beads. Incubation of the tagged RNA probes and
protein lysates encourages the formation of RNPs. After several washes to
remove nonspecific binding, the RNP complex is pulled down and the proteins
that interact with the RNA sequence of interest are subsequently identified using
mass spectrometry
Using Bead-Tagged RNAs to Identify RBPs
243