Science of Foundation (LY19C020002), Hangzhou Normal University (Sino-EU Plant RNA Signaling S&T Platform Initiative
9995C5021841101), and a NSF Plant Genome Research Program
award no. DBI-0820659 to DH.
References
1. Lucas WJ, Groover A, Lichtenberger R,
Furuta K, Yadav SR, Helariutta Y, He XQ,
Fukuda H, Kang J, Brady SM, Patrick JW,
Sperry J, Yoshida A, Lo ´ pez-Milla ´n AF, Grusak
MA, Kachroo P (2013) The plant vascular system: evolution, development and functions. J
Integr Plant Biol 55:294–388
2. Asano T, Masumura T, Kusano H, Kikuchi S,
Kurita A, Shimada H, Kadowaki K (2002)
Construction of a specialized cDNA library
from plant cells isolated by laser capture microdissection: Toward comprehensive analysis of
the genes expressed in the rice phloem. Plant
J 32:401–408
3. Vilaine F, Palauqui JC, Amselem J, Kusiak C,
Lemoine R, Dinant S (2003) Towards deciphering phloem: a transcriptome analysis of
the phloem of Apium graveolens. Plant J
36:67–81
4. Omid A, Keilin T, Glass A, Leshkowitz D, Wolf
S (2007) Characterization of phloem-sap transcription profile in melon plants. J Exp Bot
58:3645–3656
5. Deeken R, Ache P, Kajahn I, Klinkenberg J,
Bringmann G, Hedrich R (2008) Identification
of Arabidopsis thaliana phloem RNAs provides
a search criterion for phloem-based transcripts
hidden in complex datasets of microarray
experiments. Plant J 55:746–759
6. Gaupels F, Buhtz A, Knauer T, Deshmukh S,
Waller F, van Bel AJE, Kogel KH, Kehr J
(2008) Adaptation of aphid stylectomy for analyses of proteins and mRNAs in barley phloem
sap. J Exp Bot 59:3297–3306
7. Lough TJ, Lucas WJ (2006) Integrative plant
biology: role of phloem long-distance macromolecular trafficking. Annu Rev Plant Biol
57:203–232
8. Notaguchi M, Higashiyama T, Suzuki T
(2015) Identification of mRNAs that move
over long distances using a RNA-Seq analysis
of Arabidopsis/Nicotiana benthamiana heterografts. Plant Cell Physiol 56:311–321
9. Thieme CJ, Rojas-Triana M, Stecyk E,
Schudoma C, Zhang W, Yang L,
Min ˜ambres M, Walther D, Schulze WX,
Paz-Ares J, Scheible WR, Kragler F (2015)
Endogenous Arabidopsis messenger RNAs
transported to distant tissues. Nat Plants
1:15025
10. Banerjee AK, Chatterjee M, Yu Y, Suh SG,
Miller WA, Hannapel DJ (2006) Dynamics of
a mobile RNA of potato involved in a longdistance signaling pathway. Plant Cell
18:3443–3457
11. Ghate TH, Sharma P, Khondare KR, Hannapel
DJ, Banerjee AK (2017) The mobile RNAs,
StBEL11 and StBEL29, suppress growth of
tubers in potato. Plant Mol Biol 93:563–578
12. Mahajan A, Bhogle S, Kang IH, Hannapel DJ,
Banerjee AK (2012) The mRNA of a
Knotted1-like transcription factor of potato is
phloem mobile. Plant Mol Biol 79:595–608
13. Haywood V, Yu TS, Huang NC, Lucas WJ
(2005) Phloem long-distance trafficking of
gibberellic acid-insensitive RNA regulates leaf
development. Plant J 42:49–68
14. Kim M, Canio W, Kessler S, Sinha N (2001)
Developmental changes due to long distance
movement of a homeobox fusion transcript in
tomato. Science 293:287–289
15. Notaguchi M, Wolf S, Lucas WJ (2012)
Phloem-mobile Aux/IAA transcripts target to
the root tip and modify root architecture. J
Integr Plant Biol 54:760–772
16. Li C, Gu M, Shi N, Zhang H, Yang X,
Osman T, Liu Y, Wang H, Vatish M,
Jackson S, Hong Y (2011) Mobile FT mRNA
contributes to the systemic florigen signalling
in floral induction. Sci Rep 1:73
17. Lu KJ, Huang NC, Liu YS, Lu CA, Yu TS
(2012) Long-distance movement of Arabidopsis flowering locus T RNA participates in systemic floral regulation. RNA Biol 9:653–662
18. Huang NC, Jane WN, Chen J, Yu TS (2012)
Arabidopsis centroradialis homologue acts systemically to inhibit floral initiation in Arabidopsis. Plant J 72:175–184
19. Baulcombe D, Gilbert J, Goulden M, Ko ¨hm B,
Cruz SS (1994) Molecular biology of resistance to potato virus X in potato. Biochem
Soc Symp 60:207–218
20. Lico C, Benvenuto E, Baschieri S (2015) The
two-faced potato virus X: from plant pathogen
to smart nanoparticle. Front Plant Sci 6:1009
PVX-Based RNA Movement Assay
193
9995C5021841101), and a NSF Plant Genome Research Program
award no. DBI-0820659 to DH.
References
1. Lucas WJ, Groover A, Lichtenberger R,
Furuta K, Yadav SR, Helariutta Y, He XQ,
Fukuda H, Kang J, Brady SM, Patrick JW,
Sperry J, Yoshida A, Lo ´ pez-Milla ´n AF, Grusak
MA, Kachroo P (2013) The plant vascular system: evolution, development and functions. J
Integr Plant Biol 55:294–388
2. Asano T, Masumura T, Kusano H, Kikuchi S,
Kurita A, Shimada H, Kadowaki K (2002)
Construction of a specialized cDNA library
from plant cells isolated by laser capture microdissection: Toward comprehensive analysis of
the genes expressed in the rice phloem. Plant
J 32:401–408
3. Vilaine F, Palauqui JC, Amselem J, Kusiak C,
Lemoine R, Dinant S (2003) Towards deciphering phloem: a transcriptome analysis of
the phloem of Apium graveolens. Plant J
36:67–81
4. Omid A, Keilin T, Glass A, Leshkowitz D, Wolf
S (2007) Characterization of phloem-sap transcription profile in melon plants. J Exp Bot
58:3645–3656
5. Deeken R, Ache P, Kajahn I, Klinkenberg J,
Bringmann G, Hedrich R (2008) Identification
of Arabidopsis thaliana phloem RNAs provides
a search criterion for phloem-based transcripts
hidden in complex datasets of microarray
experiments. Plant J 55:746–759
6. Gaupels F, Buhtz A, Knauer T, Deshmukh S,
Waller F, van Bel AJE, Kogel KH, Kehr J
(2008) Adaptation of aphid stylectomy for analyses of proteins and mRNAs in barley phloem
sap. J Exp Bot 59:3297–3306
7. Lough TJ, Lucas WJ (2006) Integrative plant
biology: role of phloem long-distance macromolecular trafficking. Annu Rev Plant Biol
57:203–232
8. Notaguchi M, Higashiyama T, Suzuki T
(2015) Identification of mRNAs that move
over long distances using a RNA-Seq analysis
of Arabidopsis/Nicotiana benthamiana heterografts. Plant Cell Physiol 56:311–321
9. Thieme CJ, Rojas-Triana M, Stecyk E,
Schudoma C, Zhang W, Yang L,
Min ˜ambres M, Walther D, Schulze WX,
Paz-Ares J, Scheible WR, Kragler F (2015)
Endogenous Arabidopsis messenger RNAs
transported to distant tissues. Nat Plants
1:15025
10. Banerjee AK, Chatterjee M, Yu Y, Suh SG,
Miller WA, Hannapel DJ (2006) Dynamics of
a mobile RNA of potato involved in a longdistance signaling pathway. Plant Cell
18:3443–3457
11. Ghate TH, Sharma P, Khondare KR, Hannapel
DJ, Banerjee AK (2017) The mobile RNAs,
StBEL11 and StBEL29, suppress growth of
tubers in potato. Plant Mol Biol 93:563–578
12. Mahajan A, Bhogle S, Kang IH, Hannapel DJ,
Banerjee AK (2012) The mRNA of a
Knotted1-like transcription factor of potato is
phloem mobile. Plant Mol Biol 79:595–608
13. Haywood V, Yu TS, Huang NC, Lucas WJ
(2005) Phloem long-distance trafficking of
gibberellic acid-insensitive RNA regulates leaf
development. Plant J 42:49–68
14. Kim M, Canio W, Kessler S, Sinha N (2001)
Developmental changes due to long distance
movement of a homeobox fusion transcript in
tomato. Science 293:287–289
15. Notaguchi M, Wolf S, Lucas WJ (2012)
Phloem-mobile Aux/IAA transcripts target to
the root tip and modify root architecture. J
Integr Plant Biol 54:760–772
16. Li C, Gu M, Shi N, Zhang H, Yang X,
Osman T, Liu Y, Wang H, Vatish M,
Jackson S, Hong Y (2011) Mobile FT mRNA
contributes to the systemic florigen signalling
in floral induction. Sci Rep 1:73
17. Lu KJ, Huang NC, Liu YS, Lu CA, Yu TS
(2012) Long-distance movement of Arabidopsis flowering locus T RNA participates in systemic floral regulation. RNA Biol 9:653–662
18. Huang NC, Jane WN, Chen J, Yu TS (2012)
Arabidopsis centroradialis homologue acts systemically to inhibit floral initiation in Arabidopsis. Plant J 72:175–184
19. Baulcombe D, Gilbert J, Goulden M, Ko ¨hm B,
Cruz SS (1994) Molecular biology of resistance to potato virus X in potato. Biochem
Soc Symp 60:207–218
20. Lico C, Benvenuto E, Baschieri S (2015) The
two-faced potato virus X: from plant pathogen
to smart nanoparticle. Front Plant Sci 6:1009
PVX-Based RNA Movement Assay
193