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(2018) Broccoli fluorets: split aptamers as a
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org/10.1021/acschembio.7b00332
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immunoassay: light-up RNA aptamer-based
immunoassay using in vitro transcription.
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Ding T, Gao H, Liu D, Deng R, Li J (2019) A
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Chem Commun (Camb). https://doi.org/
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WW (2015) Fluorescent monitoring of RNA
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sb5000725
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activity. Talanta 199:21–26. https://doi.org/
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115. Svensen N, Jaffrey SR (2016) Fluorescent
RNA aptamers as a tool to study
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23(3):415–425. https://doi.org/10.1016/j.
chembiol.2015.11.018
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(2012) Fluorescence imaging of cellular
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Modular aptameric sensors. J Am Chem Soc
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1021/ja032013t
118. Sharma S, Zaveri A, Visweswariah SS, Krishnan Y (2014) A fluorescent nucleic acid nanodevice quantitatively images elevated cyclic
adenosine monophosphate in membranebound
compartments.
Small
10
(21):4276–4280. https://doi.org/10.1002/
smll.201400833
119. Nakayama S, Luo Y, Zhou J, Dayie TK, Sintim HO (2012) Nanomolar fluorescent
detection of c-di-GMP using a modular aptamer strategy. Chem Commun (Camb) 48
(72):9059–9061. https://doi.org/10.1039/
c2cc34379g
120. Kellenberger CA, Wilson SC, Sales-Lee J,
Hammond MC (2013) RNA-based fluorescent biosensors for live cell imaging of second
messengers cyclic di-GMP and cyclic
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(13):4906–4909. https://doi.org/10.1021/
ja311960g
121. Kellenberger CA, Chen C, Whiteley AT, Portnoy DA, Hammond MC (2015) RNA-based
fluorescent biosensors for live cell imaging of
second messenger cyclic di-AMP. J Am Chem
Soc 137(20):6432–6435. https://doi.org/
10.1021/jacs.5b00275
122. Kellenberger CA, Wilson SC, Hickey SF,
Gonzalez TL, Su Y, Hallberg ZF, Brewer
TF, Iavarone AT, Carlson HK, Hsieh YF,
Hammond MC (2015) GEMM-I riboswitches from Geobacter sense the bacterial
second messenger cyclic AMP-GMP. Proc
Natl Acad Sci U S A 112(17):5383–5388.
https://doi.org/10.1073/pnas.
1419328112
Light-Up RNA Aptamers
101
https://doi.org/10.3390/nano9030378
105. Torelli E, Kozyra JW, Gu JY, Stimming U,
Piantanida L, Voitchovsky K, Krasnogor N
(2018) Isothermal folding of a light-up
bio-orthogonal RNA origami nanoribbon.
Sci Rep 8(1):6989. https://doi.org/10.
1038/s41598-018-25270-6
106. Chandler M, Lyalina T, Halman J, Rackley L,
Lee L, Dang D, Ke W, Sajja S, Woods S,
Acharya S, Baumgarten E, Christopher J,
Elshalia E, Hrebien G, Kublank K, Saleh S,
Stallings B, Tafere M, Striplin C, Afonin KA
(2018) Broccoli fluorets: split aptamers as a
user-friendly fluorescent toolkit for dynamic
RNA nanotechnology. Molecules 23(12).
https://doi.org/10.3390/
molecules23123178
107. Shechner DM, Hacisuleyman E, Younger ST,
Rinn JL (2015) Multiplexable, locus-specific
targeting of long RNAs with CRISPRDisplay. Nat Methods 12(7):664–670.
https://doi.org/10.1038/nmeth.3433
108. Roszyk L, Kollenda S, Hennig S (2017)
Using a specific RNA-protein interaction to
quench the fluorescent RNA Spinach. ACS
Chem Biol 12(12):2958–2964. https://doi.
org/10.1021/acschembio.7b00332
109. Song W, Strack RL, Jaffrey SR (2013) Imaging bacterial protein expression using genetically encoded RNA sensors. Nat Methods 10
(9):873–875.
https://doi.org/10.1038/
nmeth.2568
110. Ying ZM, Xiao HY, Tang H, Yu RQ, Jiang JH
(2018) Light-up RNA aptamer enabled labelfree protein detection via a proximity induced
transcription assay. Chem Commun (Camb)
54(64):8877–8880.
https://doi.org/10.
1039/c8cc04498h
111. Sim J, Byun JY, Shin YB (2019) Transcription
immunoassay: light-up RNA aptamer-based
immunoassay using in vitro transcription.
Chem
Commun
(Camb)
55
(25):3618–3621. https://doi.org/10.1039/
c9cc00514e
112. Sheng L, Lu Y, Deng S, Liao X, Zhang K,
Ding T, Gao H, Liu D, Deng R, Li J (2019) A
transcription aptasensor: amplified, label-free
and culture-independent detection of foodborne pathogens via light-up RNA aptamers.
Chem Commun (Camb). https://doi.org/
10.1039/c9cc05036a
113. Rogers TA, Andrews GE, Jaeger L, Grabow
WW (2015) Fluorescent monitoring of RNA
assembly and processing using the splitspinach aptamer. ACS Synth Biol 4
(2):162–166.
https://doi.org/10.1021/
sb5000725
114. Zhou Y, Shen S, Lau C, Lu J (2019) A conformational switch-based fluorescent biosensor for homogeneous detection of telomerase
activity. Talanta 199:21–26. https://doi.org/
10.1016/j.talanta.2019.02.018
115. Svensen N, Jaffrey SR (2016) Fluorescent
RNA aptamers as a tool to study
RNA-modifying enzymes. Cell Chem Biol
23(3):415–425. https://doi.org/10.1016/j.
chembiol.2015.11.018
116. Paige JS, Nguyen-Duc T, Song W, Jaffrey SR
(2012) Fluorescence imaging of cellular
metabolites with RNA. Science 335
(6073):1194. https://doi.org/10.1126/sci
ence.1218298
117. Stojanovic MN, Kolpashchikov DM (2004)
Modular aptameric sensors. J Am Chem Soc
126(30):9266–9270.
https://doi.org/10.
1021/ja032013t
118. Sharma S, Zaveri A, Visweswariah SS, Krishnan Y (2014) A fluorescent nucleic acid nanodevice quantitatively images elevated cyclic
adenosine monophosphate in membranebound
compartments.
Small
10
(21):4276–4280. https://doi.org/10.1002/
smll.201400833
119. Nakayama S, Luo Y, Zhou J, Dayie TK, Sintim HO (2012) Nanomolar fluorescent
detection of c-di-GMP using a modular aptamer strategy. Chem Commun (Camb) 48
(72):9059–9061. https://doi.org/10.1039/
c2cc34379g
120. Kellenberger CA, Wilson SC, Sales-Lee J,
Hammond MC (2013) RNA-based fluorescent biosensors for live cell imaging of second
messengers cyclic di-GMP and cyclic
AMP-GMP. J Am Chem Soc 135
(13):4906–4909. https://doi.org/10.1021/
ja311960g
121. Kellenberger CA, Chen C, Whiteley AT, Portnoy DA, Hammond MC (2015) RNA-based
fluorescent biosensors for live cell imaging of
second messenger cyclic di-AMP. J Am Chem
Soc 137(20):6432–6435. https://doi.org/
10.1021/jacs.5b00275
122. Kellenberger CA, Wilson SC, Hickey SF,
Gonzalez TL, Su Y, Hallberg ZF, Brewer
TF, Iavarone AT, Carlson HK, Hsieh YF,
Hammond MC (2015) GEMM-I riboswitches from Geobacter sense the bacterial
second messenger cyclic AMP-GMP. Proc
Natl Acad Sci U S A 112(17):5383–5388.
https://doi.org/10.1073/pnas.
1419328112
Light-Up RNA Aptamers
101