xi
Acknowledgments
We thank many people who inspired and made this book possible, including past
and current students, research associates, and collaborators. Bess Ward and Rachel
Foster encouraged JPZ on the idea for the book. Kendra Turk-Kubo, M. Rosie
Gradoville, and Francisco Cornejo-Castillo reviewed and provided suggestions for
the manuscript. Chris Schvarcz, M. Rosie Gradoville, Kyoko Hagino, Britt Henke,
Helena Höglander, Joe Montoya, Ana Cabello-Pérez, Lasse Riemann, Ajit
Subramaniam, and Angelicque White provided images and graphics. William
Newton gave welcome advice and Dennis Dean provided a helpful review of Chap.
2 and graphics. We also thank Hans Paerl, Anne Dekas, Adam Martiny, and four
anonymous reviewers for their valuable and thoughtful comments. JPZ thanks Mary
Maxon for inspiration and support in writing the book. DGC thanks his spouse,
Linda Duguay, for her forbearance with his distractedness during the preparation of
the manuscript.
DGC thanks his early mentors and advisors, Barrie Taylor and Ed Carpenter, for
setting him on his path studying marine N 2 fixation and fellow senior grad student
Ron Oremland for training him on gas chromatography and how to sing while you
work. JPZ would like to thank his previous advisors who started his studies on
marine N 2 fixation and molecular biology: coauthor D.G. Capone, P. G. Falkowski,
and E. J. Carpenter. We would both like to thank the many people who have
contributed to nitrogen fixation research, particularly in the early years and cruises,
including Brad Bebout, Birgitta Bergman, Ed Carpenter, the late John Gallon, Joe
Montoya, Hans Paerl, Tracy Villareal, Lucas Stal, Ajit Subramaniam, and
M. Wyman. JPZ would like to thank the many collaborators on N 2 fixation from the
C-MORE and SCOPE projects, including Matt Church, Dave Karl, Ricardo Letelier,
Angelicque White, and Sam Wilson. JPZ is indebted to the late Don Comb
(1927–2020) of New England Biolabs (NEB) for encouraging and supporting the
early studies on the molecular biology of Trichodesmium and thanks
L. A. McReynolds (NEB) for advising and guiding the development of nitrogenase
PCR that has been so important in learning about uncultivated N 2 -fixing
microorganisms in the environment. We are grateful to all of our students, postdocs,
and lab members over the years who contributed to work on marine N 2 fixation.
Acknowledgments
We thank many people who inspired and made this book possible, including past
and current students, research associates, and collaborators. Bess Ward and Rachel
Foster encouraged JPZ on the idea for the book. Kendra Turk-Kubo, M. Rosie
Gradoville, and Francisco Cornejo-Castillo reviewed and provided suggestions for
the manuscript. Chris Schvarcz, M. Rosie Gradoville, Kyoko Hagino, Britt Henke,
Helena Höglander, Joe Montoya, Ana Cabello-Pérez, Lasse Riemann, Ajit
Subramaniam, and Angelicque White provided images and graphics. William
Newton gave welcome advice and Dennis Dean provided a helpful review of Chap.
2 and graphics. We also thank Hans Paerl, Anne Dekas, Adam Martiny, and four
anonymous reviewers for their valuable and thoughtful comments. JPZ thanks Mary
Maxon for inspiration and support in writing the book. DGC thanks his spouse,
Linda Duguay, for her forbearance with his distractedness during the preparation of
the manuscript.
DGC thanks his early mentors and advisors, Barrie Taylor and Ed Carpenter, for
setting him on his path studying marine N 2 fixation and fellow senior grad student
Ron Oremland for training him on gas chromatography and how to sing while you
work. JPZ would like to thank his previous advisors who started his studies on
marine N 2 fixation and molecular biology: coauthor D.G. Capone, P. G. Falkowski,
and E. J. Carpenter. We would both like to thank the many people who have
contributed to nitrogen fixation research, particularly in the early years and cruises,
including Brad Bebout, Birgitta Bergman, Ed Carpenter, the late John Gallon, Joe
Montoya, Hans Paerl, Tracy Villareal, Lucas Stal, Ajit Subramaniam, and
M. Wyman. JPZ would like to thank the many collaborators on N 2 fixation from the
C-MORE and SCOPE projects, including Matt Church, Dave Karl, Ricardo Letelier,
Angelicque White, and Sam Wilson. JPZ is indebted to the late Don Comb
(1927–2020) of New England Biolabs (NEB) for encouraging and supporting the
early studies on the molecular biology of Trichodesmium and thanks
L. A. McReynolds (NEB) for advising and guiding the development of nitrogenase
PCR that has been so important in learning about uncultivated N 2 -fixing
microorganisms in the environment. We are grateful to all of our students, postdocs,
and lab members over the years who contributed to work on marine N 2 fixation.