Fig. 4 HPLC analysis of carotenoid substrates and apocarotenoid products.
(a) Schematic illustration of the enzymatic conversion of zeaxanthin by BCO2.
(b) HPLC trace at 420 nm of a lipid extract of a control incubation of zeaxanthin in
the absence of BCO2 enzyme. (c) HPLC trace at 420 nm of a lipid extract of an
incubation of zeaxanthin in the presence of mouse BCO2
Mammalian Carotenoid Cleavage Dioxygenases
85