the catalytic site to select for geranyl diphosphate
activity. Biochemistry 39:15316–15321
21. Nagel R, Bernholz C, Vranova ´ E, Kos ˇuth J,
Bergau N, Ludwig S, Wessjohann L,
Gershenzon J, Tissier A, Schmidt A (2015)
Arabidopsis thaliana isoprenyl diphosphate
synthases produce the C 25 intermediate, geranylfarnesyl diphosphate. Plant J 84:847–859.
https://doi.org/10.1111/tpj.13064
22. Wang C, Chen Q, Fan D, Li J, Wang G, Zhang
P (2016) Structural analyses of short-chain prenyltransferases identify an evolutionarily conserved GFPPS clade in Brassicaceae plants.
Mol Plant 9:195–204. https://doi.org/10.
1016/j.molp.2015.10.010
23. Cunillera N, Arro ´ M, Delourme D, Karst F,
Boronat A, Ferrer A (1996) Arabidopsis thaliana contains two differentially expressed
farnesyl-diphosphate synthase genes. J Biol
Chem 271:7774–7780. https://doi.org/10.
1074/jbc.271.13.7774
24. Masferrer A, Arro ´ M, Manzano D, Schaller H,
Ferna ´ndez-Busquets
X,
Moncalea ´n
P,
Ferna ´ndez B, Cunillera N, Boronat A, Ferrer
A (2002) Overexpression of Arabidopsis thaliana farnesyl diphosphate synthase (FPS1S) in
transgenic Arabidopsis induces a cell death/
senescence-like response and reduced cytokinin
levels. Plant J 30:123–132
25. Chang T-H, Hsieh F-L, Ko T-P, Teng K-H,
Liang P-H, Wang AH-J (2010) Structure of a
heterotetrameric
geranyl
pyrophosphate
synthase from mint (Mentha piperita) reveals
Intersubunit
regulation.
Plant
Cell
22:454–467. https://doi.org/10.1105/tpc.
109.071738
26. Kim OT, Bang KH, Jung SJ, Kim YC, Hyun
DY, Kim SH, Cha SW (2010) Molecular characterization of ginseng farnesyl diphosphate
synthase gene and its up-regulation by methyl
jasmonate. Biol Plant 54:47–53. https://doi.
org/10.1007/s10535-010-0007-1
27. Schmidt A, W€ achtler B, Temp U, Krekling T,
Se ´guin A, Gershenzon J (2010) A bifunctional
geranyl and geranylgeranyl diphosphate
synthase is involved in terpene oleoresin formation in Picea abies. Plant Physiol 152:639–655.
https://doi.org/10.1104/pp.109.144691
28. Arro ´ M, Manzano D, Ferrer A (2014) Farnesyl
diphosphate synthase assay. Methods Mol Biol
1153:41–53. https://doi.org/10.1007/9781-4939-0606-2_4
29. Henneman L, van Cruchten AG, Denis SW,
Amolins MW, Placzek AT, Gibbs RA,
Kulik W, Waterham HR (2008) Detection of
nonsterol isoprenoids by HPLC-MS/MS. Anal
Biochem 383:18–24. https://doi.org/10.
1016/j.ab.2008.08.023
30. Nagel R, Gershenzon J, Schmidt A (2012)
Nonradioactive assay for detecting isoprenyl
diphosphate synthase activity in crude plant
extracts using liquid chromatography coupled
with tandem mass spectrometry. Anal Biochem
422:433–438. https://doi.org/10.1016/j.ab.
2011.12.037
31. Ruiz-Sola MA ´ , Barja MV, Manzano D,
Llorente B, Schipper B, Beekwilder J,
Rodriguez-Concepcion M (2016) A single
gene encodes two differentially targeted geranylgeranyl diphosphate synthase isoforms.
Plant Physiol 172:1393–1402. https://doi.
org/10.1104/pp.16.01392
32. Ruiz-Sola MA ´ , Coman D, Beck G, Barja MV,
Colinas M, Graf A, Welsch R, Ru ¨timann P,
Bu ¨hlmann P, Bigler L, Gruissem W, Rodrı ´guez-Concepcio ´ n M, Vranova ´ E (2016) Arabidopsis
GERANYLGERANYL
DIPHOSPHATE SYNTHASE 11 is a hub isozyme required for the production of most
photosynthesis-related isoprenoids. New Phytol 209:252–264. https://doi.org/10.1111/
nph.13580
33. Wang G, Dixon R (2009) A heterodimeric geranyl(geranyl)diphosphate synthase from hop
(Humulus lupulus) and the evolution of monoterpene biosynthesis. Proc Natl Acad Sci U S A
106:9914–9919. https://doi.org/10.1073/
pnas.0904069106
38
M. Victoria Barja and Manuel Rodrı ´guez-Concepcio ´ n
activity. Biochemistry 39:15316–15321
21. Nagel R, Bernholz C, Vranova ´ E, Kos ˇuth J,
Bergau N, Ludwig S, Wessjohann L,
Gershenzon J, Tissier A, Schmidt A (2015)
Arabidopsis thaliana isoprenyl diphosphate
synthases produce the C 25 intermediate, geranylfarnesyl diphosphate. Plant J 84:847–859.
https://doi.org/10.1111/tpj.13064
22. Wang C, Chen Q, Fan D, Li J, Wang G, Zhang
P (2016) Structural analyses of short-chain prenyltransferases identify an evolutionarily conserved GFPPS clade in Brassicaceae plants.
Mol Plant 9:195–204. https://doi.org/10.
1016/j.molp.2015.10.010
23. Cunillera N, Arro ´ M, Delourme D, Karst F,
Boronat A, Ferrer A (1996) Arabidopsis thaliana contains two differentially expressed
farnesyl-diphosphate synthase genes. J Biol
Chem 271:7774–7780. https://doi.org/10.
1074/jbc.271.13.7774
24. Masferrer A, Arro ´ M, Manzano D, Schaller H,
Ferna ´ndez-Busquets
X,
Moncalea ´n
P,
Ferna ´ndez B, Cunillera N, Boronat A, Ferrer
A (2002) Overexpression of Arabidopsis thaliana farnesyl diphosphate synthase (FPS1S) in
transgenic Arabidopsis induces a cell death/
senescence-like response and reduced cytokinin
levels. Plant J 30:123–132
25. Chang T-H, Hsieh F-L, Ko T-P, Teng K-H,
Liang P-H, Wang AH-J (2010) Structure of a
heterotetrameric
geranyl
pyrophosphate
synthase from mint (Mentha piperita) reveals
Intersubunit
regulation.
Plant
Cell
22:454–467. https://doi.org/10.1105/tpc.
109.071738
26. Kim OT, Bang KH, Jung SJ, Kim YC, Hyun
DY, Kim SH, Cha SW (2010) Molecular characterization of ginseng farnesyl diphosphate
synthase gene and its up-regulation by methyl
jasmonate. Biol Plant 54:47–53. https://doi.
org/10.1007/s10535-010-0007-1
27. Schmidt A, W€ achtler B, Temp U, Krekling T,
Se ´guin A, Gershenzon J (2010) A bifunctional
geranyl and geranylgeranyl diphosphate
synthase is involved in terpene oleoresin formation in Picea abies. Plant Physiol 152:639–655.
https://doi.org/10.1104/pp.109.144691
28. Arro ´ M, Manzano D, Ferrer A (2014) Farnesyl
diphosphate synthase assay. Methods Mol Biol
1153:41–53. https://doi.org/10.1007/9781-4939-0606-2_4
29. Henneman L, van Cruchten AG, Denis SW,
Amolins MW, Placzek AT, Gibbs RA,
Kulik W, Waterham HR (2008) Detection of
nonsterol isoprenoids by HPLC-MS/MS. Anal
Biochem 383:18–24. https://doi.org/10.
1016/j.ab.2008.08.023
30. Nagel R, Gershenzon J, Schmidt A (2012)
Nonradioactive assay for detecting isoprenyl
diphosphate synthase activity in crude plant
extracts using liquid chromatography coupled
with tandem mass spectrometry. Anal Biochem
422:433–438. https://doi.org/10.1016/j.ab.
2011.12.037
31. Ruiz-Sola MA ´ , Barja MV, Manzano D,
Llorente B, Schipper B, Beekwilder J,
Rodriguez-Concepcion M (2016) A single
gene encodes two differentially targeted geranylgeranyl diphosphate synthase isoforms.
Plant Physiol 172:1393–1402. https://doi.
org/10.1104/pp.16.01392
32. Ruiz-Sola MA ´ , Coman D, Beck G, Barja MV,
Colinas M, Graf A, Welsch R, Ru ¨timann P,
Bu ¨hlmann P, Bigler L, Gruissem W, Rodrı ´guez-Concepcio ´ n M, Vranova ´ E (2016) Arabidopsis
GERANYLGERANYL
DIPHOSPHATE SYNTHASE 11 is a hub isozyme required for the production of most
photosynthesis-related isoprenoids. New Phytol 209:252–264. https://doi.org/10.1111/
nph.13580
33. Wang G, Dixon R (2009) A heterodimeric geranyl(geranyl)diphosphate synthase from hop
(Humulus lupulus) and the evolution of monoterpene biosynthesis. Proc Natl Acad Sci U S A
106:9914–9919. https://doi.org/10.1073/
pnas.0904069106
38
M. Victoria Barja and Manuel Rodrı ´guez-Concepcio ´ n