carotenoid/s from the C or CE supplemented NGM dishes. To
check bioassimilation of the C or CE by the nematodes, a
specific protocol has been developed and described in Subheading 3.4.
3.4 Acute Oxidative
Stress Assay with H 2 O 2
It is recommended to perform at least two independent experiments using 100 nematodes per treatment /dose in each experiment. In Fig. 1 a representative scheme of experimental design
showing the steps and final outcome is depicted (see Note 10).
Fig. 1 Flowchart of a representative experiment of carotenoid protection against acute oxidative stress by
using C. elegans as animal model. Gravid adults are placed in NGM plates (Control) or NGM supplemented with
the corresponding carotenoid or carotenoid extract, or vitamin C (positive control). A total of 100 worms per
conditions are transferred. Plates are incubated at 20
C for 7 days. Then, an oxidative stress is provoke by
H 2 O 2 (2 mM) incubation and nematodes survival is assessed under stereomicroscope
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check bioassimilation of the C or CE by the nematodes, a
specific protocol has been developed and described in Subheading 3.4.
3.4 Acute Oxidative
Stress Assay with H 2 O 2
It is recommended to perform at least two independent experiments using 100 nematodes per treatment /dose in each experiment. In Fig. 1 a representative scheme of experimental design
showing the steps and final outcome is depicted (see Note 10).
Fig. 1 Flowchart of a representative experiment of carotenoid protection against acute oxidative stress by
using C. elegans as animal model. Gravid adults are placed in NGM plates (Control) or NGM supplemented with
the corresponding carotenoid or carotenoid extract, or vitamin C (positive control). A total of 100 worms per
conditions are transferred. Plates are incubated at 20
C for 7 days. Then, an oxidative stress is provoke by
H 2 O 2 (2 mM) incubation and nematodes survival is assessed under stereomicroscope
394
Patricia Martorell et al.