transformation of tomato cotyledons (Lycopersicon esculentum L. mill.) is genotype and procedure dependent. Theor Appl Genet
106:231–238
10. Decaestecker W, Buono RA, Pfeiffer ML,
Vangheluwe N, Jourquin J, Karimi M et al
(2018) CRISPR-TSKO facilitates efficient cell
type-, tissue-, or organ-specific mutagenesis in
Arabidopsis. bioRxiv. 474981. https://doi.
org/10.1101/474981
11. Popp MW, Maquat LE (2016) Leveraging
rules of nonsense-mediated mRNA decay for
genome engineering and personalized medicine. Cell 165:1319–1322
12. Zischewski J, Fischer R, Bortesi L (2017)
Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other
sequence-specific nucleases. Biotechnol Adv
35:95–104
13. Jacobs TB, Martin GB (2016) Highthroughput CRISPR vector construction and
characterization of DNA modifications by generation of tomato hairy roots. J Vis Exp 110:
e53843
14. Fauser F, Schiml S, Puchta H (2014) Both
CRISPR/Cas-based nucleases and nickases
can be used efficiently for genome engineering
in Arabidopsis thaliana. Plant J 79:348–359
15. Ritter A, In ˜igo S, Ferna ´ndez-Calvo P, Heyndrickx KS, Dhondt S, Shi H et al (2017) The
transcriptional repressor complex FRS7-FRS12
regulates flowering time and growth in Arabidopsis. Nat Commun 8:15235
16. Pauwels L, De Clercq R, Goossens J, In ˜igo S,
Williams C, Ron M et al (2018) A dual sgRNA
approach for functional genomics in Arabidopsis thaliana. G3 Genes 8:2603–2615
17. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV,
Nguyen NT, Zheng Z et al (2015) Engineered
CRISPR-Cas9 nucleases with altered PAM specificities. Nature 523:481–485
18. Houbaert A, Zhang C, Tiwari M, Wang K, de
Marcos Serrano A, Savatin DV et al (2018)
POLAR-guided signalling complex assembly
and localization drive asymmetric cell division.
Nature 563:574–578
CRISPR-Cas-Mediated Gene Knockout
341
106:231–238
10. Decaestecker W, Buono RA, Pfeiffer ML,
Vangheluwe N, Jourquin J, Karimi M et al
(2018) CRISPR-TSKO facilitates efficient cell
type-, tissue-, or organ-specific mutagenesis in
Arabidopsis. bioRxiv. 474981. https://doi.
org/10.1101/474981
11. Popp MW, Maquat LE (2016) Leveraging
rules of nonsense-mediated mRNA decay for
genome engineering and personalized medicine. Cell 165:1319–1322
12. Zischewski J, Fischer R, Bortesi L (2017)
Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other
sequence-specific nucleases. Biotechnol Adv
35:95–104
13. Jacobs TB, Martin GB (2016) Highthroughput CRISPR vector construction and
characterization of DNA modifications by generation of tomato hairy roots. J Vis Exp 110:
e53843
14. Fauser F, Schiml S, Puchta H (2014) Both
CRISPR/Cas-based nucleases and nickases
can be used efficiently for genome engineering
in Arabidopsis thaliana. Plant J 79:348–359
15. Ritter A, In ˜igo S, Ferna ´ndez-Calvo P, Heyndrickx KS, Dhondt S, Shi H et al (2017) The
transcriptional repressor complex FRS7-FRS12
regulates flowering time and growth in Arabidopsis. Nat Commun 8:15235
16. Pauwels L, De Clercq R, Goossens J, In ˜igo S,
Williams C, Ron M et al (2018) A dual sgRNA
approach for functional genomics in Arabidopsis thaliana. G3 Genes 8:2603–2615
17. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV,
Nguyen NT, Zheng Z et al (2015) Engineered
CRISPR-Cas9 nucleases with altered PAM specificities. Nature 523:481–485
18. Houbaert A, Zhang C, Tiwari M, Wang K, de
Marcos Serrano A, Savatin DV et al (2018)
POLAR-guided signalling complex assembly
and localization drive asymmetric cell division.
Nature 563:574–578
CRISPR-Cas-Mediated Gene Knockout
341