carotenoid biosynthesis in plants using a viral
vector. Sci Rep 7:41645. https://doi.org/10.
1038/srep41645
6. Aragone ´s V, Pe ´rez-de-Castro A, Cordero T,
Cebolla-Cornejo J, Lo ´ pez C, Pico ´ B, Daro `s
JA (2019) A Watermelon mosaic virus clone
tagged with the yellow visual maker phytoene
synthase facilitates scoring infectivity in melon
breeding programs. Eur J Plant Pathol
153:317. https://doi.org/10.1007/s10658018-01621-x
7. Wylie SJ, Adams M, Chalam C, Kreuze J,
Lo ´ pez-Moya JJ, Ohshima K, Praveen S,
Rabenstein F, Stenger D, Wang A, Zerbini
FM, Ictv Report C (2017) ICTV Virus Taxonomy Profile: Potyviridae. J Gen Virol 98
(3):352–354. https://doi.org/10.1099/jgv.
0.000740
8. Revers F, Garcı ´a JA (2015) Molecular biology
of potyviruses. Adv Virus Res 92:101–199.
https://doi.org/10.1016/bs.aivir.2014.11.
006
9. Bedoya L, Martı ´nez F, Rubio L, Daro ` s JA
(2010) Simultaneous equimolar expression of
multiple proteins in plants from a disarmed
potyvirus
vector.
J
Biotechnol
150
(2):268–275.
https://doi.org/10.1016/j.
jbiotec.2010.08.006
10. Majer E, Salvador Z, Zwart MP, Willemsen A,
Elena SF, Daro ` s JA (2014) Relocation of the
NIb gene in the tobacco etch potyvirus
genome. J Virol 88(8):4586–4590. https://
doi.org/10.1128/JVI.03336-13
11. Carrington JC, Haldeman R, Dolja VV,
Restrepo-Hartwig MA (1993) Internal cleavage and trans-proteolytic activities of the
VPg-proteinase (NIa) of tobacco etch potyvirus in vivo. J Virol 67(12):6995–7000
12. Majer E, Navarro JA, Daro ` s JA (2015) A potyvirus vector efficiently targets recombinant
proteins to chloroplasts, mitochondria and
nuclei in plant cells when expressed at the
amino terminus of the polyprotein. Biotechnol
J. https://doi.org/10.1002/biot.201500042
13. Li XH, Carrington JC (1995) Complementation of tobacco etch potyvirus mutants by
active RNA polymerase expressed in transgenic
cells. Proc Natl Acad Sci U S A 92(2):457–461
14. Martı ´ M, Diretto G, Aragone ´s V, Ahrazem O,
Go ´ mez-Go ´ mez L, Daro ` s JA (2019) Efficient
production of saffron crocins and picrocrocin
in Nicotiana benthamiana using a virus-driven
system. Submitted
15. Thole V, Worland B, Snape JW, Vain P (2007)
The pCLEAN dual binary vector system for
Agrobacterium-mediated plant transformation.
Plant Physiol 145(4):1211–1219
16. Gibson DG, Young L, Chuang RY, Venter JC,
Hutchison CA 3rd, Smith HO (2009) Enzymatic assembly of DNA molecules up to several
hundred
kilobases.
Nat
Methods
6
(5):343–345.
https://doi.org/10.1038/
nmeth.1318
312
Jose ´ -Antonio Daro ` s
vector. Sci Rep 7:41645. https://doi.org/10.
1038/srep41645
6. Aragone ´s V, Pe ´rez-de-Castro A, Cordero T,
Cebolla-Cornejo J, Lo ´ pez C, Pico ´ B, Daro `s
JA (2019) A Watermelon mosaic virus clone
tagged with the yellow visual maker phytoene
synthase facilitates scoring infectivity in melon
breeding programs. Eur J Plant Pathol
153:317. https://doi.org/10.1007/s10658018-01621-x
7. Wylie SJ, Adams M, Chalam C, Kreuze J,
Lo ´ pez-Moya JJ, Ohshima K, Praveen S,
Rabenstein F, Stenger D, Wang A, Zerbini
FM, Ictv Report C (2017) ICTV Virus Taxonomy Profile: Potyviridae. J Gen Virol 98
(3):352–354. https://doi.org/10.1099/jgv.
0.000740
8. Revers F, Garcı ´a JA (2015) Molecular biology
of potyviruses. Adv Virus Res 92:101–199.
https://doi.org/10.1016/bs.aivir.2014.11.
006
9. Bedoya L, Martı ´nez F, Rubio L, Daro ` s JA
(2010) Simultaneous equimolar expression of
multiple proteins in plants from a disarmed
potyvirus
vector.
J
Biotechnol
150
(2):268–275.
https://doi.org/10.1016/j.
jbiotec.2010.08.006
10. Majer E, Salvador Z, Zwart MP, Willemsen A,
Elena SF, Daro ` s JA (2014) Relocation of the
NIb gene in the tobacco etch potyvirus
genome. J Virol 88(8):4586–4590. https://
doi.org/10.1128/JVI.03336-13
11. Carrington JC, Haldeman R, Dolja VV,
Restrepo-Hartwig MA (1993) Internal cleavage and trans-proteolytic activities of the
VPg-proteinase (NIa) of tobacco etch potyvirus in vivo. J Virol 67(12):6995–7000
12. Majer E, Navarro JA, Daro ` s JA (2015) A potyvirus vector efficiently targets recombinant
proteins to chloroplasts, mitochondria and
nuclei in plant cells when expressed at the
amino terminus of the polyprotein. Biotechnol
J. https://doi.org/10.1002/biot.201500042
13. Li XH, Carrington JC (1995) Complementation of tobacco etch potyvirus mutants by
active RNA polymerase expressed in transgenic
cells. Proc Natl Acad Sci U S A 92(2):457–461
14. Martı ´ M, Diretto G, Aragone ´s V, Ahrazem O,
Go ´ mez-Go ´ mez L, Daro ` s JA (2019) Efficient
production of saffron crocins and picrocrocin
in Nicotiana benthamiana using a virus-driven
system. Submitted
15. Thole V, Worland B, Snape JW, Vain P (2007)
The pCLEAN dual binary vector system for
Agrobacterium-mediated plant transformation.
Plant Physiol 145(4):1211–1219
16. Gibson DG, Young L, Chuang RY, Venter JC,
Hutchison CA 3rd, Smith HO (2009) Enzymatic assembly of DNA molecules up to several
hundred
kilobases.
Nat
Methods
6
(5):343–345.
https://doi.org/10.1038/
nmeth.1318
312
Jose ´ -Antonio Daro ` s