1. Clearing solution 1: 10% (w:v) potassium hydroxide (KOH).
2. Clearing solution 2: 2% (v:v) acetic acid in water.
3. Staining solution: 5% Lamy black ink (see Note 1) and 2%
acetic acid.
Mycorradicins
COOR 2
R 1 OOC
O
O
Gly
α-ionols
Fig. 1 General structure of the apocarotenoids C 13 α-ionol glycosides
(blumenols) and C 14 mycorradicin derivatives, specifically induced in
mycorrhizal plants
Negative
contacts
Positive
contacts
Fig. 2 Scheme showing the gridline intersect method for quantification of AM fungal colonization in
mycorrhizal roots. Based on the method described by Giovannetti and Mosse in 1980 [11]
AM Symbiosis Quantification
295
2. Clearing solution 2: 2% (v:v) acetic acid in water.
3. Staining solution: 5% Lamy black ink (see Note 1) and 2%
acetic acid.
Mycorradicins
COOR 2
R 1 OOC
O
O
Gly
α-ionols
Fig. 1 General structure of the apocarotenoids C 13 α-ionol glycosides
(blumenols) and C 14 mycorradicin derivatives, specifically induced in
mycorrhizal plants
Negative
contacts
Positive
contacts
Fig. 2 Scheme showing the gridline intersect method for quantification of AM fungal colonization in
mycorrhizal roots. Based on the method described by Giovannetti and Mosse in 1980 [11]
AM Symbiosis Quantification
295