0533). AB is member of the Spanish Carotenoid Network (CaRed)
funded by the Spanish Ministry of Economy and Competitiveness
(Grants BIO2015-71703-REDT and BIO2017-90877-REDT).
References
1. Egea I, Barsan C, Bian W, Purgatto E,
Latche ´ A, Chervin C, Bouzayen M, Pech JC
(2010) Chromoplast differentiation: current
status and perspectives. Plant Cell Physiol
51:1601–1611
2. Li L, Yuan H (2013) Chromoplast biogenesis
and carotenoid accumulation. Arch Biochem
Biophys 539:102–109
3. Li L, Yuan H, Zeng Y, Xu Q (2016) Plastids
and carotenoid accumulation. Subcell Biochem
79:273–293
4. Vishnevetsky M, Ovadis M, Vainstein A (1999)
Carotenoid sequestration in plants: the role of
carotenoid-associated proteins. Trends Plant
Sci 4:232–235
5. Rodriguez-Concepcion M, Avalos J, Bonet
ML, Boronat A, Gomez-Gomez L, HorneroMendez D, Limon MC, Mele ´ndez-Martı ´nez
AJ, Olmedilla-Alonso B, Palou A, Ribot J,
Rodrigo MJ, Zacarias L, Zhu C (2018) A
global perspective on carotenoids: metabolism,
biotechnology, and benefits for nutrition and
health. Prog Lipid Res 70:62–93
6. Lado J, Zacarı ´as L, Rodrigo MJ (2016) Regulation of carotenoid biosynthesis during fruit
development. Subcell Biochem 79:161–198
7. Sun T, Yuan H, Cao H, Yazdani M, Tadmor Y,
Li L (2018) Carotenoid metabolism in plants:
the role of plastids. Mol Plant 11:58–74
8. Bathgate B, Purton ME, Grierson D, Goodenough PW (1985) Plastid changes during the
conversion of chloroplasts to chromoplasts in
ripening tomatoes. Planta 165:197–204
9. Cheung AY, McNellis T, Piekos B (1993)
Maintenance of chloroplast components during chromoplast differentiation in the tomato
mutant
green
flesh.
Plant
Physiol
101:1223–1229
10. Rosso SW (1968) The ultrastructure of chromoplast development in red tomatoes. J Ultrastruct Res 25:307–322
11. Van Wijk KJ, Kessler F (2017) Plastoglobuli:
plastid microcompartments with integrated
functions in metabolism, plastid developmental
transitions, and environmental adaptation.
Annu Rev Plant Biol 68:253–289
12. Ytterberg AJ, Peltier J-B, van Wijk KJ (2006)
Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for
differential accumulation of metabolic
enzymes. Plant Physiol 140:984–997
13. Zeng Y, Du J, Wang L, Pan Z, Xu Q, Xiao S,
Deng X (2015) A comprehensive analysis of
chromoplast differentiation reveals complex
protein changes associated with plastoglobule
biogenesis and remodeling of protein systems
in sweet orange flesh. Plant Physiol
168:1648–1665
14. Barsan C, Sanchez-Bel P, Rombaldi C, Egea I,
Rossignol M, Kuntz M, Zouine M, Latche ´ A,
Bouzayen M, Pech JC (2010) Characteristics
of the tomato chromoplast revealed by proteomic analysis. J Exp Bot 61:2413–2431
15. Suzuki M, Takahashi S, Kondo T, Dohra H,
Ito Y, Kiriiwa Y, Hayashi M, Kamiya S, Kato M,
Fujiwara M, Fukao Y, Kobayashi M, Nagata N,
Motohashi R (2015) Plastid proteomic analysis
in tomato fruit development. PLoS One 10(9):
e0137266
16. Vidi PA, Kanwischer M, Baginsky S, Austin JR,
Csucs G, Do ¨rmann P, Kessler F, Bre ´he ´lin C
(2006) Tocopherol cyclase (VTE1) localization
and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles. J Biol Chem
281:11225–11234
17. Angaman DM, Petrizzo R, Herna ´ndez-Gras F,
Romero-Segura C, Pateraki I, Busquets M,
Boronat A (2012) Precursor uptake assays and
metabolic analyses in isolated tomato fruit
chromoplasts. Plant Methods 8:1
18. Herna ´ndez-Gras F, Petrizzo P, Pateraki I,
Renato M, Angaman M, Azco ´ n-Bieto J, Boronat A (2014) Isolation of tomato fruit chromoplasts and determination of ATP levels.
Bio-Protocol 4(15):e1192. http://www.bioprotocol.org/e1192
19. Rodrı ´guez-Amaya DB (2010) Quantitative
analysis, in vitro assessment of bioavailability
and antioxidant activity of food carotenoids—
a review. J Food Compos Anal 23:726–740
Chromoplast Fractionation
197
funded by the Spanish Ministry of Economy and Competitiveness
(Grants BIO2015-71703-REDT and BIO2017-90877-REDT).
References
1. Egea I, Barsan C, Bian W, Purgatto E,
Latche ´ A, Chervin C, Bouzayen M, Pech JC
(2010) Chromoplast differentiation: current
status and perspectives. Plant Cell Physiol
51:1601–1611
2. Li L, Yuan H (2013) Chromoplast biogenesis
and carotenoid accumulation. Arch Biochem
Biophys 539:102–109
3. Li L, Yuan H, Zeng Y, Xu Q (2016) Plastids
and carotenoid accumulation. Subcell Biochem
79:273–293
4. Vishnevetsky M, Ovadis M, Vainstein A (1999)
Carotenoid sequestration in plants: the role of
carotenoid-associated proteins. Trends Plant
Sci 4:232–235
5. Rodriguez-Concepcion M, Avalos J, Bonet
ML, Boronat A, Gomez-Gomez L, HorneroMendez D, Limon MC, Mele ´ndez-Martı ´nez
AJ, Olmedilla-Alonso B, Palou A, Ribot J,
Rodrigo MJ, Zacarias L, Zhu C (2018) A
global perspective on carotenoids: metabolism,
biotechnology, and benefits for nutrition and
health. Prog Lipid Res 70:62–93
6. Lado J, Zacarı ´as L, Rodrigo MJ (2016) Regulation of carotenoid biosynthesis during fruit
development. Subcell Biochem 79:161–198
7. Sun T, Yuan H, Cao H, Yazdani M, Tadmor Y,
Li L (2018) Carotenoid metabolism in plants:
the role of plastids. Mol Plant 11:58–74
8. Bathgate B, Purton ME, Grierson D, Goodenough PW (1985) Plastid changes during the
conversion of chloroplasts to chromoplasts in
ripening tomatoes. Planta 165:197–204
9. Cheung AY, McNellis T, Piekos B (1993)
Maintenance of chloroplast components during chromoplast differentiation in the tomato
mutant
green
flesh.
Plant
Physiol
101:1223–1229
10. Rosso SW (1968) The ultrastructure of chromoplast development in red tomatoes. J Ultrastruct Res 25:307–322
11. Van Wijk KJ, Kessler F (2017) Plastoglobuli:
plastid microcompartments with integrated
functions in metabolism, plastid developmental
transitions, and environmental adaptation.
Annu Rev Plant Biol 68:253–289
12. Ytterberg AJ, Peltier J-B, van Wijk KJ (2006)
Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for
differential accumulation of metabolic
enzymes. Plant Physiol 140:984–997
13. Zeng Y, Du J, Wang L, Pan Z, Xu Q, Xiao S,
Deng X (2015) A comprehensive analysis of
chromoplast differentiation reveals complex
protein changes associated with plastoglobule
biogenesis and remodeling of protein systems
in sweet orange flesh. Plant Physiol
168:1648–1665
14. Barsan C, Sanchez-Bel P, Rombaldi C, Egea I,
Rossignol M, Kuntz M, Zouine M, Latche ´ A,
Bouzayen M, Pech JC (2010) Characteristics
of the tomato chromoplast revealed by proteomic analysis. J Exp Bot 61:2413–2431
15. Suzuki M, Takahashi S, Kondo T, Dohra H,
Ito Y, Kiriiwa Y, Hayashi M, Kamiya S, Kato M,
Fujiwara M, Fukao Y, Kobayashi M, Nagata N,
Motohashi R (2015) Plastid proteomic analysis
in tomato fruit development. PLoS One 10(9):
e0137266
16. Vidi PA, Kanwischer M, Baginsky S, Austin JR,
Csucs G, Do ¨rmann P, Kessler F, Bre ´he ´lin C
(2006) Tocopherol cyclase (VTE1) localization
and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles. J Biol Chem
281:11225–11234
17. Angaman DM, Petrizzo R, Herna ´ndez-Gras F,
Romero-Segura C, Pateraki I, Busquets M,
Boronat A (2012) Precursor uptake assays and
metabolic analyses in isolated tomato fruit
chromoplasts. Plant Methods 8:1
18. Herna ´ndez-Gras F, Petrizzo P, Pateraki I,
Renato M, Angaman M, Azco ´ n-Bieto J, Boronat A (2014) Isolation of tomato fruit chromoplasts and determination of ATP levels.
Bio-Protocol 4(15):e1192. http://www.bioprotocol.org/e1192
19. Rodrı ´guez-Amaya DB (2010) Quantitative
analysis, in vitro assessment of bioavailability
and antioxidant activity of food carotenoids—
a review. J Food Compos Anal 23:726–740
Chromoplast Fractionation
197