C30 peaks
C18 peaks
18.5
18.75
19
19.25
19.5
19.75
20
20.25
20.5min
0
20
40
60
80
100
120
140
mAU (400nm)
dc-ζc
tc-ζc
ζc-1
ζc
9c-ζc
380
402
426
ζc
332
348
368
16.8
17
17.2
17.4
17.6
17.8
18min
5
10
15
20
25
30
mAU (348nm)
Phf -1
Phf-2
Phf-3
9,15dc-Phf
Phf
min
12
12.5
13
13.5
14
14.5
15
15.5
16
0
10
20
30
40
50
Phy-1
Phy-2
Phy-3
mAU (286nm)
60
286
15c-Phy
Phy
31
31.5
32min
mAU (286nm)
0
5
10
15
20
25
min
32.4
32.6
32.8
15c-Phy
+
Phy-1;3
33
0
10
20
30
40
50
60
mAU (400nm)
tc-ζc
ζc-1
dc-ζc
9c-ζc
ζc
mAU (348nm)
31.8
32
32.2
32.4
0
2
4
6
8
10
12
9,15dc-Phf
+
Phf-1;3
min
Fig. 2 Comparison of C18 and C30 columns for cis/trans carotenoid identification. Separation of cis-carotene
isomers was performed more effectively and with a higher resolution on a C30 column rather than C18. HPLC
peaks were collected from 7 day-old dark-grown cotyledons of Arabidopsis ziso (zic1-3) mutant. Abbreviations: mAU, milli-Absorbance Units. For the full nomenclature of carotenoid abbreviations; see Table 1
152
Yagiz Alagoz et al.