– The quantitative/qualitative analysis of carotenoids and other
pigments.
– The monitoring of quantitative/qualitative changes in pigments
as a result of diverse processes (postharvest treatments, industrial
processing, storage).
– The evaluation of stages of maturity on the basis of the changes
in the pigments contents,
– The grading of foods according to their quality, since color is an
important quality attribute and some carotenoids have nutritional relevance.
More information about the diverse types of studies harnessing
UV-Vis spectroscopic information to obtain information about
carotenoid contents can be found in dedicated revisions of the
literature [4, 7, 12].
2 Materials
2.1
Spectrophotometry
(Transmission
Measurement)
1. Glass cuvettes (2, 5, and 10 mm path length).
2. Sample filtration system: Syringe (13 mm diameter), filter
holder and filters (2.0 μm pore).
3. Centrifuge.
4. Plastic tubes.
5. UV–visible scanning spectrophotometer (e.g., HP8452 Hewlett-Packard).
6. CROMALAB Software [32].
2.2
Spectroradiometry
(Reflection
Measurement)
1. Glass cuvettes (10 mm path length) for liquid samples.
2. Spectroradiometer (e.g., CAS 140 B Instrument Systems).
3. External lamp.
4. Top 100 telescope optical probe.
5. Tamron zoom.
6. Cabin with grey walls (L∗ ¼ 50) (surround).
7. Pressed barium sulfate plate as white reference (background).
8. Software IS-SPECWIN.
2.3 Digital Image
Analysis (Reflection
Measurement)
1. “DigiEye” imaging system, comprised by a cabin, a digital
camera and a CPU.
2. “DigiEye” software.
3. “Digitizer” certified colour chart.
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Paula Mapelli-Brahm et al.
pigments.
– The monitoring of quantitative/qualitative changes in pigments
as a result of diverse processes (postharvest treatments, industrial
processing, storage).
– The evaluation of stages of maturity on the basis of the changes
in the pigments contents,
– The grading of foods according to their quality, since color is an
important quality attribute and some carotenoids have nutritional relevance.
More information about the diverse types of studies harnessing
UV-Vis spectroscopic information to obtain information about
carotenoid contents can be found in dedicated revisions of the
literature [4, 7, 12].
2 Materials
2.1
Spectrophotometry
(Transmission
Measurement)
1. Glass cuvettes (2, 5, and 10 mm path length).
2. Sample filtration system: Syringe (13 mm diameter), filter
holder and filters (2.0 μm pore).
3. Centrifuge.
4. Plastic tubes.
5. UV–visible scanning spectrophotometer (e.g., HP8452 Hewlett-Packard).
6. CROMALAB Software [32].
2.2
Spectroradiometry
(Reflection
Measurement)
1. Glass cuvettes (10 mm path length) for liquid samples.
2. Spectroradiometer (e.g., CAS 140 B Instrument Systems).
3. External lamp.
4. Top 100 telescope optical probe.
5. Tamron zoom.
6. Cabin with grey walls (L∗ ¼ 50) (surround).
7. Pressed barium sulfate plate as white reference (background).
8. Software IS-SPECWIN.
2.3 Digital Image
Analysis (Reflection
Measurement)
1. “DigiEye” imaging system, comprised by a cabin, a digital
camera and a CPU.
2. “DigiEye” software.
3. “Digitizer” certified colour chart.
110
Paula Mapelli-Brahm et al.