Top Med Chem (2020) 34: 1–32
DOI: 10.1007/7355_2019_86
© Springer Nature Switzerland AG 2019
Published online: 29 February 2020
Fluorescence Molecular Imaging
of Medicinal Chemistry in Cancer
Jie Tian, Yang Du, Chu Tang, and Yu An
Contents
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2 Imaging Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2.1 Fluorophores . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2.2 Targeted Ligand of Fluorescent Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3 Imaging Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.1 Photon Propagation Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.2 Forward Problem-Solving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.3 Inverse Problem-Solving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4 Medical Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 Identification of Therapeutic Targets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.2 Candidate Drug Screening . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.3 Tracking the Drug Biodistribution and Metabolism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
J. Tian (*)
CAS Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and
Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing,
China
Beijing Key Laboratory of Molecular Imaging, Beijing, China
University of Chinese Academy of Sciences, Beijing, China
Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Beihang
University, Beijing, China
Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education, School
of Life Science and Technology, Xidian University, Xi’an, Shaanxi, China
e-mail: jie.tian@ia.ac.cn; tian@ieee.org; http://www.3dmed.net
Y. Du and Y. An
CAS Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and
Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing,
China
Beijing Key Laboratory of Molecular Imaging, Beijing, China
University of Chinese Academy of Sciences, Beijing, China
C. Tang
Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education, School
of Life Science and Technology, Xidian University, Xi’an, Shaanxi, China
DOI: 10.1007/7355_2019_86
© Springer Nature Switzerland AG 2019
Published online: 29 February 2020
Fluorescence Molecular Imaging
of Medicinal Chemistry in Cancer
Jie Tian, Yang Du, Chu Tang, and Yu An
Contents
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2 Imaging Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2.1 Fluorophores . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2.2 Targeted Ligand of Fluorescent Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3 Imaging Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.1 Photon Propagation Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.2 Forward Problem-Solving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.3 Inverse Problem-Solving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4 Medical Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 Identification of Therapeutic Targets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.2 Candidate Drug Screening . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.3 Tracking the Drug Biodistribution and Metabolism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
J. Tian (*)
CAS Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and
Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing,
China
Beijing Key Laboratory of Molecular Imaging, Beijing, China
University of Chinese Academy of Sciences, Beijing, China
Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Beihang
University, Beijing, China
Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education, School
of Life Science and Technology, Xidian University, Xi’an, Shaanxi, China
e-mail: jie.tian@ia.ac.cn; tian@ieee.org; http://www.3dmed.net
Y. Du and Y. An
CAS Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and
Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing,
China
Beijing Key Laboratory of Molecular Imaging, Beijing, China
University of Chinese Academy of Sciences, Beijing, China
C. Tang
Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education, School
of Life Science and Technology, Xidian University, Xi’an, Shaanxi, China