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© Springer Nature B.V. 2020
G. Sindona et al. (eds.), Toxic Chemical and Biological Agents, NATO Science
for Peace and Security Series A: Chemistry and Biology,
https://doi.org/10.1007/978-94-024-2041-8_28
Chapter 28
Evaluation of Surface Second Harmonic
Generation SSHG for Detection
of Antibody-Antigen Interactions
Abdel Mohsen Soliman
Abstract Surface Second Harmonic Generation (SSHG) is an optical non-linear
analytical technique that has been applied to various biological and non-biological
systems. Its application to biological systems has been limited to imaging and studies of molecular structures. However, its potential as a label-free method to detect
bio-molecular interactions was not thoroughly investigated. This study explored,
probed and evaluated the potential of SSHG as a viable biosensing technique.
The present study is undertaken to investigate the application of SSHG technique
as a viable biosensing technique. This work probed immunoassays of a protein antigen (Ag), namely bovine serum albumin (BSA). The Ag-Ab interactions were
detected using (ELISA). BSA was selected to represent natural non-glycoslated
proteins. The optimization processes were trial and error experimentation with various Ag and its Primary Antibody (PAb) concentrations. Application of SSHG spectroscopy on these immunoassays and comparing them with the reference method,
i.e. ELISA.
Keywords SSHG · BSA · Laminin · PAb · Biosensor · ELISA
28.1 Materials and Methods
28.1.1 Bovine Serum Albumin (BSA) Model
BSA was used as an Ag and purified rabbit anti-BSA was used as PAb.
A. M. Soliman (*)
National Research Centre of Egypt, Cairo, Egypt