233
associated with inducing germination of C. difficile spores [4], has been shown at
high intensity in infected luminal contents while lacking in control tissue.
Distinctions between the epithelium and luminal contents were clearly demarcated
in imaging data, with confidence that this demarcation will continue in infected
samples.
Future work will apply a similar workflow to murine C. difficile infection models
for small molecule and elemental analysis. We anticipate the generation of data
from multiple imaging modalities with similar, robust qualities as those from control murine samples. Acquired data from control and infection models will then be
compared, deducing molecular changes associated with C. difficile colonization of
the gastrointestinal tract.
19.4 Novel Aspect
Imaging MS technology can be utilized to map molecular and elemental interactions in the gastrointestinal tract caused by C. difficile infection.
References
1. Abt MC, McKenney PT, Pamer EG (2016) Clostridium difficile colitis: pathogenesis and host
defence. Nat Rev Microbiol 14:609–620
2. Fischetti Vincent A, Novick Richard P, Ferretti Joseph J, Portnoy Daniel A, Rood JI (2000)
Gram-positive pathogens, pp 551–563
3. Cornett DS et al (2007) MALDI imaging mass spectrometry: molecular snapshots of biochemical systems. Nat Methods 4:828–833
4. Sagar NM, Cree IA, Covington JA, Arasaradnam RP (2015) The interplay of the gut microbiome, bile acids, and volatile organic compounds. Gastroenterol Res Pract
19 Mapping Molecular Interactions in the Clostridioides difficile Infected…
associated with inducing germination of C. difficile spores [4], has been shown at
high intensity in infected luminal contents while lacking in control tissue.
Distinctions between the epithelium and luminal contents were clearly demarcated
in imaging data, with confidence that this demarcation will continue in infected
samples.
Future work will apply a similar workflow to murine C. difficile infection models
for small molecule and elemental analysis. We anticipate the generation of data
from multiple imaging modalities with similar, robust qualities as those from control murine samples. Acquired data from control and infection models will then be
compared, deducing molecular changes associated with C. difficile colonization of
the gastrointestinal tract.
19.4 Novel Aspect
Imaging MS technology can be utilized to map molecular and elemental interactions in the gastrointestinal tract caused by C. difficile infection.
References
1. Abt MC, McKenney PT, Pamer EG (2016) Clostridium difficile colitis: pathogenesis and host
defence. Nat Rev Microbiol 14:609–620
2. Fischetti Vincent A, Novick Richard P, Ferretti Joseph J, Portnoy Daniel A, Rood JI (2000)
Gram-positive pathogens, pp 551–563
3. Cornett DS et al (2007) MALDI imaging mass spectrometry: molecular snapshots of biochemical systems. Nat Methods 4:828–833
4. Sagar NM, Cree IA, Covington JA, Arasaradnam RP (2015) The interplay of the gut microbiome, bile acids, and volatile organic compounds. Gastroenterol Res Pract
19 Mapping Molecular Interactions in the Clostridioides difficile Infected…