218
the corticosteroids response: steroid-Dependent, (D), steroid-Sensitive (S) and
steroid- Resistant (R).
Firstly, a characteristic SDS-PAGE protein profile emerged to be associated with
each class, which preserves its peculiarity also when compared to UE protein content of non-INS patients (orthostatic proteinuria, hereditary tubulopathies) and
healthy age-matched controls. Through a hierarchical clustering, we confirmed the
imprints provided by the gel analysis: the UE of INS patients divided the cohort
according to the clinical classifications.
Secondly, by immunoblotting, we also pinpointed different levels of specific glomerular membrane proteins (PgP, CD10, Podocin), described as involved in the INS
development. Moreover, we observed that each protein above mentioned showed a
major band located at the expected molecular weight (MW) and at least one other
located at the higher MW, both significantly varied among the three classes, especially in the case of CD10.
These evidences confirmed the feasibility to use a UE approach to intercept the
pathophysiological differences underlying the response to therapy. At the present
time, we are proceeding in performing mass spectrometric analysis to more in depth
comprehend the emerged differences in the UE proteome of the pilot cohort.
Simultaneously, we are enlarging the sample size in order to develop a robust classification model, able to use UE as predictive parameter of long-term prognosis to
treatment response.
References
1. Morrison EE, Bailey M, Dear JW (2016) Renal extracellular vesicles: from physiology to clinical application. J Physiol (20):5735–5748
2. Pasini A, Benetti E, Conti G, Ghio L, Lepore M, Massella L, Molino D, Peruzzi L, Emma F,
Fede C, Trivelli A, Maringhini S, Materassi M, Messina G, Montini G, Murer L, Pecoraro C,
Pennesi M (2017) The Italian Society for Pediatric Nephrology (SINePe) consensus document
on the management of nephrotic syndrome in children: part I – diagnosis and treatment of the
first episode and the first relapse. Italian J Pediatr 43:41
E. Barigazzi et al.
the corticosteroids response: steroid-Dependent, (D), steroid-Sensitive (S) and
steroid- Resistant (R).
Firstly, a characteristic SDS-PAGE protein profile emerged to be associated with
each class, which preserves its peculiarity also when compared to UE protein content of non-INS patients (orthostatic proteinuria, hereditary tubulopathies) and
healthy age-matched controls. Through a hierarchical clustering, we confirmed the
imprints provided by the gel analysis: the UE of INS patients divided the cohort
according to the clinical classifications.
Secondly, by immunoblotting, we also pinpointed different levels of specific glomerular membrane proteins (PgP, CD10, Podocin), described as involved in the INS
development. Moreover, we observed that each protein above mentioned showed a
major band located at the expected molecular weight (MW) and at least one other
located at the higher MW, both significantly varied among the three classes, especially in the case of CD10.
These evidences confirmed the feasibility to use a UE approach to intercept the
pathophysiological differences underlying the response to therapy. At the present
time, we are proceeding in performing mass spectrometric analysis to more in depth
comprehend the emerged differences in the UE proteome of the pilot cohort.
Simultaneously, we are enlarging the sample size in order to develop a robust classification model, able to use UE as predictive parameter of long-term prognosis to
treatment response.
References
1. Morrison EE, Bailey M, Dear JW (2016) Renal extracellular vesicles: from physiology to clinical application. J Physiol (20):5735–5748
2. Pasini A, Benetti E, Conti G, Ghio L, Lepore M, Massella L, Molino D, Peruzzi L, Emma F,
Fede C, Trivelli A, Maringhini S, Materassi M, Messina G, Montini G, Murer L, Pecoraro C,
Pennesi M (2017) The Italian Society for Pediatric Nephrology (SINePe) consensus document
on the management of nephrotic syndrome in children: part I – diagnosis and treatment of the
first episode and the first relapse. Italian J Pediatr 43:41
E. Barigazzi et al.