201
digests. Thus, the CID-MS/MS analysis of theses glycated peptides allowed to discover 17 glycation sites, localized on the following lysine residues: Lys 65, Lys 75,
Lys 88, Lys 100, Lys 117, Lys 151, Lys 183, Lys 197, Lys 256, Lys 266, Lys 304,
Lys 309, Lys 336, Lys 374, Lys 420, Lys 455 and Lys 495.
Table 11.2 Tryptic glycopeptides identified of the bovine serum albumin protein by LC-ESIQqTOF-MS/MS analysis of the hapten-BSA glycoconjugate [69]
Precursor
ion
m/z
Deviation Missed
(Charge)
Mr (expt) Mr (calc) Da
Cleavage Peptide
729.8826
(+2)
1457.7506 1457.7389 0.0117
1
KHK
*
P (Lys 559)
733.3170
(+2)
1464.6194 1464.7698 −0.1504 1
QIK
*
K (Lys 547)
770.3757
(+2)
1538.7369 1538.7338 0.0031
1
ADEK
*
K (Lys 155)
807.9138
(+2)
1613.8129 1613.7964 0.0165
0
K
* FWGK (Lys 156)
828.0811
(+3)
2481.2213 2481.2005 0.0208
2
LKECCDK
*
PLLEK (Lys 304)
832.7770
(+3)
2495.3092 2495.3146 −0.0054 1
LK
*
HLVDEPQNLIK (Lys 401)
863.7965
(+3)
2588.3677 2588.3572 0.0105
0
K
* VPQVSTPTLVEVSR (Lys 437)
883.9655
(+2)
1765.9164 1765.9085 0.0079
1
SLGK
* VGTR (Lys 455)
922.0941
(+3)
2763.2605 2763.2493 0.0112
1
LAK
*
EYEATLEECCAK (Lys 374)
969.5086
(+2)
1937.0026 1936.9868 0.0158
1
TPVSEK
* VTK (Lys 495)
970.4900
(+2)
1938.9738 1938.9772 −0.0034 1
EK
*
VLTSSAR (Lys 211)
976.0173
(+2)
1950.0201 1950.0085 0.0116
1
ALK
*
AWSVAR (Lys 235)
990.4710
(+3)
2968.3911 2968.3886 0.0025
2
LK
*
PDPNTLCDEFKADEK (Lys
140)
1073.0144
(+2)
2144,0143 2144.0082 0.0061
1
CASIQK
* FGER (Lys 228)
1058.4652
(+2)
2114.9159 2114.9123 0.0036
1
CCTK
*
PESER (Lys 463)
1208.5857
(+2)
2415.1569 2415.1284 0.0285
1
VTK
*
CCTESLVNR (Lys 498)
1160.2172
(+3)
3477.6297 3477.6385 −0.0088 1
QNCDQFEK
* LGEYGFQNALIVR
(Lys 420)
1169.5978
(+2)
2337.1810 2337.1583 0.0227
1
GACLLPK
*
IETMR (Lys 204)
11 Defense Against Biological Terrorism: Vaccines and Their Characterizations
digests. Thus, the CID-MS/MS analysis of theses glycated peptides allowed to discover 17 glycation sites, localized on the following lysine residues: Lys 65, Lys 75,
Lys 88, Lys 100, Lys 117, Lys 151, Lys 183, Lys 197, Lys 256, Lys 266, Lys 304,
Lys 309, Lys 336, Lys 374, Lys 420, Lys 455 and Lys 495.
Table 11.2 Tryptic glycopeptides identified of the bovine serum albumin protein by LC-ESIQqTOF-MS/MS analysis of the hapten-BSA glycoconjugate [69]
Precursor
ion
m/z
Deviation Missed
(Charge)
Mr (expt) Mr (calc) Da
Cleavage Peptide
729.8826
(+2)
1457.7506 1457.7389 0.0117
1
KHK
*
P (Lys 559)
733.3170
(+2)
1464.6194 1464.7698 −0.1504 1
QIK
*
K (Lys 547)
770.3757
(+2)
1538.7369 1538.7338 0.0031
1
ADEK
*
K (Lys 155)
807.9138
(+2)
1613.8129 1613.7964 0.0165
0
K
* FWGK (Lys 156)
828.0811
(+3)
2481.2213 2481.2005 0.0208
2
LKECCDK
*
PLLEK (Lys 304)
832.7770
(+3)
2495.3092 2495.3146 −0.0054 1
LK
*
HLVDEPQNLIK (Lys 401)
863.7965
(+3)
2588.3677 2588.3572 0.0105
0
K
* VPQVSTPTLVEVSR (Lys 437)
883.9655
(+2)
1765.9164 1765.9085 0.0079
1
SLGK
* VGTR (Lys 455)
922.0941
(+3)
2763.2605 2763.2493 0.0112
1
LAK
*
EYEATLEECCAK (Lys 374)
969.5086
(+2)
1937.0026 1936.9868 0.0158
1
TPVSEK
* VTK (Lys 495)
970.4900
(+2)
1938.9738 1938.9772 −0.0034 1
EK
*
VLTSSAR (Lys 211)
976.0173
(+2)
1950.0201 1950.0085 0.0116
1
ALK
*
AWSVAR (Lys 235)
990.4710
(+3)
2968.3911 2968.3886 0.0025
2
LK
*
PDPNTLCDEFKADEK (Lys
140)
1073.0144
(+2)
2144,0143 2144.0082 0.0061
1
CASIQK
* FGER (Lys 228)
1058.4652
(+2)
2114.9159 2114.9123 0.0036
1
CCTK
*
PESER (Lys 463)
1208.5857
(+2)
2415.1569 2415.1284 0.0285
1
VTK
*
CCTESLVNR (Lys 498)
1160.2172
(+3)
3477.6297 3477.6385 −0.0088 1
QNCDQFEK
* LGEYGFQNALIVR
(Lys 420)
1169.5978
(+2)
2337.1810 2337.1583 0.0227
1
GACLLPK
*
IETMR (Lys 204)
11 Defense Against Biological Terrorism: Vaccines and Their Characterizations