193
oligosaccharide- BSA glycoconjugates: (B) GlcNAc-BSA (carbohydrate- BSA ratio
(n) = 8.4); (C) lactose-BSA (n = 3.5); (D) lactose-BSA (n = 8.2); (E) Fucal-2Galfl
l-3[Fucal-4]GlcNAc- BSA (n = 11); (F) Fucal-2Fucal- 3GalNAc-BSA (n = 13). The
MALDI-TOF-MS spectrum of BSA was used as a calibration standard. Then the
analysis allowed to reveal the average carbohydrate-BSA ratios for the hapten-BSA
neoglycoconjugates (Fig. 11.4).
Fig. 11.4 MALDI-TOF spectra of: (a) BSA calibration standard; (b) GlcNAc-BSA (n = 8.4); (c)
lactose-BSA (n = 3.5); (d) lactose-BSA (n = 8.2); (e) Fucal-2Galfl l-3[Fucal-4]GlcNAc-BSA
(n = 11); (f) Fucal-2Fucal-3GalNAc-BSA (n = 13) [58]
11 Defense Against Biological Terrorism: Vaccines and Their Characterizations
oligosaccharide- BSA glycoconjugates: (B) GlcNAc-BSA (carbohydrate- BSA ratio
(n) = 8.4); (C) lactose-BSA (n = 3.5); (D) lactose-BSA (n = 8.2); (E) Fucal-2Galfl
l-3[Fucal-4]GlcNAc- BSA (n = 11); (F) Fucal-2Fucal- 3GalNAc-BSA (n = 13). The
MALDI-TOF-MS spectrum of BSA was used as a calibration standard. Then the
analysis allowed to reveal the average carbohydrate-BSA ratios for the hapten-BSA
neoglycoconjugates (Fig. 11.4).
Fig. 11.4 MALDI-TOF spectra of: (a) BSA calibration standard; (b) GlcNAc-BSA (n = 8.4); (c)
lactose-BSA (n = 3.5); (d) lactose-BSA (n = 8.2); (e) Fucal-2Galfl l-3[Fucal-4]GlcNAc-BSA
(n = 11); (f) Fucal-2Fucal-3GalNAc-BSA (n = 13) [58]
11 Defense Against Biological Terrorism: Vaccines and Their Characterizations