Table 1 Biohydrogen production by consolidated bioprocessing of lignocellulosic biomass using pure, mixed, and co-cultures
Sr no.
Microorganism
Substrate and
pretreatment
Experimental
conditions
H 2 yield
References
A. Pure culture microbes
1.
Clostridium sartagoforme FZ11
Untreated corn
stalk powder
15 g/L, batch
mode, 35 °C
87.2 mL/g corn stalk
Zhang et al.
(2015)
2.
Clostridium thermocellum 1313
Alkali pretreated
spent mushroom
compost (SMC)
4% w/v SMC,
batch mode,
55 °C
0.74 mol/mol
glucose
Lin et al. (2017)
3.
Clostridium butyricum FS3
Untreated corn
stalk powder
10 g/L, batch
mode, 35 °C
92.9 mL/g substrate
Song et al. (2014)
4.
Caldicellulosiruptor saccharolyticus
DSM8903
Untreated switch
grass
(SWG) powder
3% (w/v) SWG,
batch mode,
65 °C
11.2 mmol/g SWG
Talluri et al.
(2013)
5.
Thermoanaerobacterium sp. strain F6
Untreated sugar
bagasse
30 g/L, batch
mode, 60 °C
1.81 mmol/g
substrate
Jiang et al. (2019)
6.
Thermoanaerobacterium sp. strain F6
Untreated corn
Cob
30 g/L, batch
mode, 60 °C
2.07 mmol/g
substrate
Jiang et al. (2019)
7.
Thermoanaerobacterium
thermosaccharolyticum DD32
Untreated chipped
corn stalk
5 g/L, batch
mode, 55 °C
6.38 mmol/g
substrate
Sheng et al.
(2015)
8.
E. coli ZH-4
Untreated corn
straw
15 g/L, batch
mode, 37 °C
4.71 mL/g substrate
Pang et al. (2017)
B. Mixed culture microbes
9.
Cow dung compost
Acid pretreated
wheat straw wastes
25 g/L, batch
mode, 36 °C
68.1 mL H 2 /g total
volatile solid
Fan (2006)
10
Cellobiose acclimated sludge
Untreated
microalga,
Chlorella
sp. ESP-6
20 g/L, batch
mode, 30 °C
0.56 mmol/g
biomass
Ho et al. (2012)
11
Untreated sewage sludge
Untreated rice
straw
27 g rice straw,
batch mode,
55 °C,
0.74 mmol/g
substrate
Kim et al. (2012)
12
Geobacillus sp. WSUCF1 (aerobic)
with Thermophilic consortia isolated
from hot spring (anaerobic)
Untreated water
insoluble prairie
cordgrass
2% prepared
cordgrass, batch
mode, 60 °C
3.74 mmol/g
substrate
Bibra et al. (2018)
13.
Clostridium acetobutylicum (ATCC
824) and bovine ruminal fluid (BRF)
Acid treated agave
biomass
10% solid
loading, batch
mode, 35 °C
150 L/kg biomass
Morales-Martínez
et al. (2020)
14.
Anaerobic thermophilic bacteria
consortium BK16
Untreated cassava
pulp,
10% (w/v)
cassava pulp,
batch mode,
60 °C
23 mL/g substrate
Pason et al. (2020)
C. Coculture microbes
15.
Enterococcus consortia of wheat straw
with Epiphytic xylanolytic
Untreated raw
wheat straw
powder
2.5% total solid,
batch mode,
37 °C
79.54 mL/g wheat
straw xylose
Valdez-Vazquez
et al. (2015)
16.
Citrobacter amalonaticus NRBC13547
and C. cellulolyticum DSM 5812
Steam-exploded
corn stover
25 g/L, batch
mode, 121 °C
51.9 L/Kg TS
Zhang et al.
(2016)
17
C. thermosaccharolyticum DSM572
and C. thermocellum DSM7072
Untreated sweet
sorghum stalk
powder
5 g/L, batch
mode, 55 °C
5.1 mmol/g-substrate
Islam et al. (2017)
Bioconversion of Lignocellulosic Residues into Hydrogen
73
Sr no.
Microorganism
Substrate and
pretreatment
Experimental
conditions
H 2 yield
References
A. Pure culture microbes
1.
Clostridium sartagoforme FZ11
Untreated corn
stalk powder
15 g/L, batch
mode, 35 °C
87.2 mL/g corn stalk
Zhang et al.
(2015)
2.
Clostridium thermocellum 1313
Alkali pretreated
spent mushroom
compost (SMC)
4% w/v SMC,
batch mode,
55 °C
0.74 mol/mol
glucose
Lin et al. (2017)
3.
Clostridium butyricum FS3
Untreated corn
stalk powder
10 g/L, batch
mode, 35 °C
92.9 mL/g substrate
Song et al. (2014)
4.
Caldicellulosiruptor saccharolyticus
DSM8903
Untreated switch
grass
(SWG) powder
3% (w/v) SWG,
batch mode,
65 °C
11.2 mmol/g SWG
Talluri et al.
(2013)
5.
Thermoanaerobacterium sp. strain F6
Untreated sugar
bagasse
30 g/L, batch
mode, 60 °C
1.81 mmol/g
substrate
Jiang et al. (2019)
6.
Thermoanaerobacterium sp. strain F6
Untreated corn
Cob
30 g/L, batch
mode, 60 °C
2.07 mmol/g
substrate
Jiang et al. (2019)
7.
Thermoanaerobacterium
thermosaccharolyticum DD32
Untreated chipped
corn stalk
5 g/L, batch
mode, 55 °C
6.38 mmol/g
substrate
Sheng et al.
(2015)
8.
E. coli ZH-4
Untreated corn
straw
15 g/L, batch
mode, 37 °C
4.71 mL/g substrate
Pang et al. (2017)
B. Mixed culture microbes
9.
Cow dung compost
Acid pretreated
wheat straw wastes
25 g/L, batch
mode, 36 °C
68.1 mL H 2 /g total
volatile solid
Fan (2006)
10
Cellobiose acclimated sludge
Untreated
microalga,
Chlorella
sp. ESP-6
20 g/L, batch
mode, 30 °C
0.56 mmol/g
biomass
Ho et al. (2012)
11
Untreated sewage sludge
Untreated rice
straw
27 g rice straw,
batch mode,
55 °C,
0.74 mmol/g
substrate
Kim et al. (2012)
12
Geobacillus sp. WSUCF1 (aerobic)
with Thermophilic consortia isolated
from hot spring (anaerobic)
Untreated water
insoluble prairie
cordgrass
2% prepared
cordgrass, batch
mode, 60 °C
3.74 mmol/g
substrate
Bibra et al. (2018)
13.
Clostridium acetobutylicum (ATCC
824) and bovine ruminal fluid (BRF)
Acid treated agave
biomass
10% solid
loading, batch
mode, 35 °C
150 L/kg biomass
Morales-Martínez
et al. (2020)
14.
Anaerobic thermophilic bacteria
consortium BK16
Untreated cassava
pulp,
10% (w/v)
cassava pulp,
batch mode,
60 °C
23 mL/g substrate
Pason et al. (2020)
C. Coculture microbes
15.
Enterococcus consortia of wheat straw
with Epiphytic xylanolytic
Untreated raw
wheat straw
powder
2.5% total solid,
batch mode,
37 °C
79.54 mL/g wheat
straw xylose
Valdez-Vazquez
et al. (2015)
16.
Citrobacter amalonaticus NRBC13547
and C. cellulolyticum DSM 5812
Steam-exploded
corn stover
25 g/L, batch
mode, 121 °C
51.9 L/Kg TS
Zhang et al.
(2016)
17
C. thermosaccharolyticum DSM572
and C. thermocellum DSM7072
Untreated sweet
sorghum stalk
powder
5 g/L, batch
mode, 55 °C
5.1 mmol/g-substrate
Islam et al. (2017)
Bioconversion of Lignocellulosic Residues into Hydrogen
73