ruthenium red after one and three days of inoculation on original and synthetic
wounds after two days; total phenolic and flavonoid accumulation; phenylalanine
lyase activity along with percent grey mold infection germination rate. The outcomes revealed that development of disease incidence and induction lesion were
decreased with gradual increase in dextran concentration but the incidence was
slightly higher with dextran and ruthenium red combination; accumulation of total
phenolic and flavonoid compounds were higher with dextran elicitation after one
day and two days of inoculation, respectively; the phenylalanine lyase activity was
higher with dextran treatment after twelve hour of inoculation but it hits the lowest
point after two of inoculation also the rate of spore germination in presence of
B. cinerea was gradually decreased with dextran treatment. So these data confirm
the importance of dextran as biotic elicitor to strengthening the plant against grey
mold and greater accumulation of phenolic compound as well as flavonoid content
in tomato fruit (Lu et al. 2019b).
1.4.2.10 Effect of Yeast on Vincristine and Vinblastine Production
in Catharanthus roseus Plant
Maqsood et al. scientifically proved the importance yeast as biotic elicitor on the
production of vincristine and vinblastine obtained from protoplast sourced tissues
and plant of Catharanthus roseus (commonly known as periwinkle). The experiment was commenced from protoplast culture involving incubation between suspended cells and enzymes treatment (cellulose, pectinase, macroenzyme and
driselase) in MS medium followed by treatments with NAA, dichlorophenoxyacetic
acid, BA and gibberellin within the same medium to culture the callus and embryo
formation followed by elicitation using different concentrations of yeast (0.5, 1.0,
1.5 and 2.0 g/L). The proliferation, maturation and germination of vincristine and
vinblastine after different yeast elicitation. The outcomes showed that maximum
observations were observed with 1.5 and 2.0 g/L yeast concentrations, so these
elicitations were selected for enzymatic activities (catalase, superoxide dismutase,
ascorbate peroxidase and glutathione reductase) in the medium with different plant
regulators. The results showed that maximum catalase and ascorbate peroxidase
activities were observed with leaf harvested tissues with 2.0 g/L yeast in the
medium contained BA and NAA; the superoxide dismutase and glutathione peroxidase activities were observed with 2.0 g/L yeast in the medium contained BA
and NAA in embryo germination stage (Fig. 1.8). So these outcomes confirmed the
importance of yeast on production of vincristine and vinblastine in Catharanthus
roseus plant (Maqsood and Mujib 2017).
22
S. Saha and D. Pal
wounds after two days; total phenolic and flavonoid accumulation; phenylalanine
lyase activity along with percent grey mold infection germination rate. The outcomes revealed that development of disease incidence and induction lesion were
decreased with gradual increase in dextran concentration but the incidence was
slightly higher with dextran and ruthenium red combination; accumulation of total
phenolic and flavonoid compounds were higher with dextran elicitation after one
day and two days of inoculation, respectively; the phenylalanine lyase activity was
higher with dextran treatment after twelve hour of inoculation but it hits the lowest
point after two of inoculation also the rate of spore germination in presence of
B. cinerea was gradually decreased with dextran treatment. So these data confirm
the importance of dextran as biotic elicitor to strengthening the plant against grey
mold and greater accumulation of phenolic compound as well as flavonoid content
in tomato fruit (Lu et al. 2019b).
1.4.2.10 Effect of Yeast on Vincristine and Vinblastine Production
in Catharanthus roseus Plant
Maqsood et al. scientifically proved the importance yeast as biotic elicitor on the
production of vincristine and vinblastine obtained from protoplast sourced tissues
and plant of Catharanthus roseus (commonly known as periwinkle). The experiment was commenced from protoplast culture involving incubation between suspended cells and enzymes treatment (cellulose, pectinase, macroenzyme and
driselase) in MS medium followed by treatments with NAA, dichlorophenoxyacetic
acid, BA and gibberellin within the same medium to culture the callus and embryo
formation followed by elicitation using different concentrations of yeast (0.5, 1.0,
1.5 and 2.0 g/L). The proliferation, maturation and germination of vincristine and
vinblastine after different yeast elicitation. The outcomes showed that maximum
observations were observed with 1.5 and 2.0 g/L yeast concentrations, so these
elicitations were selected for enzymatic activities (catalase, superoxide dismutase,
ascorbate peroxidase and glutathione reductase) in the medium with different plant
regulators. The results showed that maximum catalase and ascorbate peroxidase
activities were observed with leaf harvested tissues with 2.0 g/L yeast in the
medium contained BA and NAA; the superoxide dismutase and glutathione peroxidase activities were observed with 2.0 g/L yeast in the medium contained BA
and NAA in embryo germination stage (Fig. 1.8). So these outcomes confirmed the
importance of yeast on production of vincristine and vinblastine in Catharanthus
roseus plant (Maqsood and Mujib 2017).
22
S. Saha and D. Pal