Table 8.1
Application of C.e in Alkd
Name of
The
alkaloid
Structure
Solvent
Diameter of column
Vt
g
Detector
References
Purine
N
N
O
NH
O
N
N
O
N
O
H
N
N N
N
O
N
O
N
20 mM borate, pH 9.2
50 cm x 50 um
10 kV
254 nm
(Mukherjee
et al. 2018)
Ephedra
OH
H
N
20 mM isoleucine, 5 mM barium hydroxide
(pH 10.0 with ammonia) methanol, 10 mM
phosphate (1:4); 10 mM borate (pH 9.0),
100 mM SDS
60 cm
 75 pm (52.5 cm 27
°C 60cm
 75pm (52.5 25 °
C 48 cm
 50 pm (26 cm
86) 30 °C
20–28
185 nm
(Chahel
et al. 2019)
Imidazole
O
O
N
N
100 mM phosphate PH 6.9, 10 mM
B-cyclodextrin
50 cm
 50 pm (40 um to
detector)
8
217 nm
(Badria and
Aboelmaaty
2019)
Oxindole
N
H
N
O
O
20 mM phosphate (PH 5.6)
57 cm
 75 pm (50 cm to
detector) 25 °C
10
254
(Kolrep
et al. 2019)
294
D. Pal and S. Mukherjee
Application of C.e in Alkd
Name of
The
alkaloid
Structure
Solvent
Diameter of column
Vt
g
Detector
References
Purine
N
N
O
NH
O
N
N
O
N
O
H
N
N N
N
O
N
O
N
20 mM borate, pH 9.2
50 cm x 50 um
10 kV
254 nm
(Mukherjee
et al. 2018)
Ephedra
OH
H
N
20 mM isoleucine, 5 mM barium hydroxide
(pH 10.0 with ammonia) methanol, 10 mM
phosphate (1:4); 10 mM borate (pH 9.0),
100 mM SDS
60 cm
 75 pm (52.5 cm 27
°C 60cm
 75pm (52.5 25 °
C 48 cm
 50 pm (26 cm
86) 30 °C
20–28
185 nm
(Chahel
et al. 2019)
Imidazole
O
O
N
N
100 mM phosphate PH 6.9, 10 mM
B-cyclodextrin
50 cm
 50 pm (40 um to
detector)
8
217 nm
(Badria and
Aboelmaaty
2019)
Oxindole
N
H
N
O
O
20 mM phosphate (PH 5.6)
57 cm
 75 pm (50 cm to
detector) 25 °C
10
254
(Kolrep
et al. 2019)
294
D. Pal and S. Mukherjee