4
our studies on the diversity of Ligularia species, root phytochemicals and evolutionary neutral DNA sequences were chosen as indices, in addition to morphological
identification and ecological observation in the field. As neutral DNA samples,
internal transcribed spacer (ITS1-5.8S-ITS2) sequences were analyzed in the ribosomal RNA gene, and, for chemical investigations, EtOH or AcOEt extracts of dried
roots were selected. The root phytochemical profiles and the ITS sequences were
examined as independent parameters to one another. To date, many sesquiterpenoids, euparin-type benzofurans, and phenylpropanoids have been isolated.
Eremophilanes, particularly furanoeremophilanes and eremophilan- 12,8- olides,
Plate 1 A typical collection site in Aba, Sichuan Province, China
Plate 2 A typical collection site in Shangri-La, Yunnan Province, China
M. Tori and C. Kuroda
our studies on the diversity of Ligularia species, root phytochemicals and evolutionary neutral DNA sequences were chosen as indices, in addition to morphological
identification and ecological observation in the field. As neutral DNA samples,
internal transcribed spacer (ITS1-5.8S-ITS2) sequences were analyzed in the ribosomal RNA gene, and, for chemical investigations, EtOH or AcOEt extracts of dried
roots were selected. The root phytochemical profiles and the ITS sequences were
examined as independent parameters to one another. To date, many sesquiterpenoids, euparin-type benzofurans, and phenylpropanoids have been isolated.
Eremophilanes, particularly furanoeremophilanes and eremophilan- 12,8- olides,
Plate 1 A typical collection site in Aba, Sichuan Province, China
Plate 2 A typical collection site in Shangri-La, Yunnan Province, China
M. Tori and C. Kuroda