Acknowledgments
This work was partly funded by a grant (LEK-02/2012) from the
Research Council of Lithuania.
References
1. Elsey D, Jameson D, Raleigh B, Cooney MJ
(2007) Fluorescent measurement of microalgal
neutral lipids. J Microbiol Methods 68:639–642
2. Gao C, Xiong W, Zhang Y, Yuan W, Wu Q
(2008) Rapid quantitation of lipid in microalgae by time-domain nuclear magnetic resonance. J Microbiol Methods 75(3):437–440
3. Mutanda T, Ramesh D, Karthikeyan S,
Kumari S, Anandraj A, Bux F (2011) Bioprospecting for hyper-lipid producing microalgal
strains for sustainable biofuel production. Bioresour Technol 102:57–70
4. Natunen K, Sepp€ al€ a J, Schwenk D, Rischer H,
Spilling K, Tamminen T (2015) Nile red staining of phytoplankton neutral lipids: speciesspecific fluorescence kinetics in various solvents. J Appl Phycol 27(3):1161–1168
5. Govender T, Ramanna L, Rawat I, Bux F
(2012) BODIPY staining, an alternative to
the Nile red fluorescence method for the evaluation of intracellular lipids in microalgae.
Bioresour Technol 91(2):321–328
6. Duan X, Li P, Li P, Xie T, Yu F, Tang B (2011)
The synthesis of polarity-sensitive fluorescent
dyes based on the BODIPY chromophore.
Dyes Pigments 89:217e222
7. Niu S-L, Ulrich G, Retailleau P, Harrowfield J, Ziessel R (2009) New insights into the solubilization of
Bodipy dyes. Tetrahedron Lett 50:3840–3844
8. Yang L, Simionescu R, Lough A, Yan H (2011)
Some observations relating to the stability of
the BODIPY fluorophore under acidic and
basic conditions. Dyes Pigments 91:264e267
9. Xu D, Gao Z, Li F, Fan X, Zhang X, Ye N,
Moua S, Liang C, Li D (2013) Detection and
quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining. Bioresour Technol
127:386–390
10. Rumin J, Bonnefond H, Saint-Jean B,
Rouxel C, Sciandra A, Bernard O, Cadoret
J-P, Bougaran G (2015) The use of fluorescent
Nile red and BODIPY for lipid measurement in
microalgae. Biotechnol Biofuels 8:42
11. Elle IC, Olsen LCB, Pultz D, Rødkær SV, Færgeman NJ (2010) Something worth dyeing
for: molecular tools for the dissection of lipid
metabolism in Caenorhabditis elegans. FEBS
Lett 584:2183–2193
12. Cooper MS, Hardin WR, Petersen TW, Cattolico RA (2010) Visualizing “green oil” in live
algal cells. J Biosci Bioeng 109(2):198–201
13. Brennan L, Blanco Ferna ´ndez A, Mostaert AS,
Owende P (2012) Enhancement of BODIPY505/515 lipid fluorescence method for
applications in biofuel directed microalgae production. J Microbiol Methods 90:137–143
14. Pereira H, Barreira L, Mozes A, Florindo C,
Polo C, Duarte CV, Custo ´ dio L, Varela J
(2011) Microplate-based high throughput
screening procedure for the isolation of lipidrich marine microalgae. Biotechnol Biofuels
4:61
15. De la Hoz SH, Ayidzoe W, Ben-Zvi A, Burrell
RE, McCaffrey WC (2012) Improving the reliability of fluorescence-based neutral lipid content measurements in microalgal cultures. Algal
Res 1:176–184
16. Doan T-TY, Obbard JP (2011) Improved Nile
red staining of Nannochloropsis sp. J Appl Phycol 23:895–901
17. Cirulis JT, Strasser BC, Scott JA, Ross GM
(2012) Optimization of staining conditions
for microalgae with three lipophilic dyes to
reduce precipitation and fluorescence variability. Cytometry A 81(7):618–626
18. Dominguez Cabanelas IT, van der Zwart M,
Kleinegris DMM, Wijffels RH, Barbosa MJ
(2016) Sorting cells of the microalga Chlorococcum littorale with increased triacylglycerol
productivity. Biotechnol Biofuels 9:183
Microalgae Lipid Staining with Fluorescent BODIPY Dye
53
This work was partly funded by a grant (LEK-02/2012) from the
Research Council of Lithuania.
References
1. Elsey D, Jameson D, Raleigh B, Cooney MJ
(2007) Fluorescent measurement of microalgal
neutral lipids. J Microbiol Methods 68:639–642
2. Gao C, Xiong W, Zhang Y, Yuan W, Wu Q
(2008) Rapid quantitation of lipid in microalgae by time-domain nuclear magnetic resonance. J Microbiol Methods 75(3):437–440
3. Mutanda T, Ramesh D, Karthikeyan S,
Kumari S, Anandraj A, Bux F (2011) Bioprospecting for hyper-lipid producing microalgal
strains for sustainable biofuel production. Bioresour Technol 102:57–70
4. Natunen K, Sepp€ al€ a J, Schwenk D, Rischer H,
Spilling K, Tamminen T (2015) Nile red staining of phytoplankton neutral lipids: speciesspecific fluorescence kinetics in various solvents. J Appl Phycol 27(3):1161–1168
5. Govender T, Ramanna L, Rawat I, Bux F
(2012) BODIPY staining, an alternative to
the Nile red fluorescence method for the evaluation of intracellular lipids in microalgae.
Bioresour Technol 91(2):321–328
6. Duan X, Li P, Li P, Xie T, Yu F, Tang B (2011)
The synthesis of polarity-sensitive fluorescent
dyes based on the BODIPY chromophore.
Dyes Pigments 89:217e222
7. Niu S-L, Ulrich G, Retailleau P, Harrowfield J, Ziessel R (2009) New insights into the solubilization of
Bodipy dyes. Tetrahedron Lett 50:3840–3844
8. Yang L, Simionescu R, Lough A, Yan H (2011)
Some observations relating to the stability of
the BODIPY fluorophore under acidic and
basic conditions. Dyes Pigments 91:264e267
9. Xu D, Gao Z, Li F, Fan X, Zhang X, Ye N,
Moua S, Liang C, Li D (2013) Detection and
quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining. Bioresour Technol
127:386–390
10. Rumin J, Bonnefond H, Saint-Jean B,
Rouxel C, Sciandra A, Bernard O, Cadoret
J-P, Bougaran G (2015) The use of fluorescent
Nile red and BODIPY for lipid measurement in
microalgae. Biotechnol Biofuels 8:42
11. Elle IC, Olsen LCB, Pultz D, Rødkær SV, Færgeman NJ (2010) Something worth dyeing
for: molecular tools for the dissection of lipid
metabolism in Caenorhabditis elegans. FEBS
Lett 584:2183–2193
12. Cooper MS, Hardin WR, Petersen TW, Cattolico RA (2010) Visualizing “green oil” in live
algal cells. J Biosci Bioeng 109(2):198–201
13. Brennan L, Blanco Ferna ´ndez A, Mostaert AS,
Owende P (2012) Enhancement of BODIPY505/515 lipid fluorescence method for
applications in biofuel directed microalgae production. J Microbiol Methods 90:137–143
14. Pereira H, Barreira L, Mozes A, Florindo C,
Polo C, Duarte CV, Custo ´ dio L, Varela J
(2011) Microplate-based high throughput
screening procedure for the isolation of lipidrich marine microalgae. Biotechnol Biofuels
4:61
15. De la Hoz SH, Ayidzoe W, Ben-Zvi A, Burrell
RE, McCaffrey WC (2012) Improving the reliability of fluorescence-based neutral lipid content measurements in microalgal cultures. Algal
Res 1:176–184
16. Doan T-TY, Obbard JP (2011) Improved Nile
red staining of Nannochloropsis sp. J Appl Phycol 23:895–901
17. Cirulis JT, Strasser BC, Scott JA, Ross GM
(2012) Optimization of staining conditions
for microalgae with three lipophilic dyes to
reduce precipitation and fluorescence variability. Cytometry A 81(7):618–626
18. Dominguez Cabanelas IT, van der Zwart M,
Kleinegris DMM, Wijffels RH, Barbosa MJ
(2016) Sorting cells of the microalga Chlorococcum littorale with increased triacylglycerol
productivity. Biotechnol Biofuels 9:183
Microalgae Lipid Staining with Fluorescent BODIPY Dye
53