essential that the chromatographic conditions are identical and
the standard compounds are used for validation of retention
times.
Acknowledgments
The authors thank Ulla Lahtinen and Airi Hyrk€ as for excellent
technical assistance in preparing extracts of algal samples.
References
1. Kumari P, Kumar M, Reddy CRK et al (2013)
Algal lipids, fatty acids and sterols. In: Dominguez H (ed) Functional ingredients from algae
for foods and nutraceuticals. Woodhead Publishing Ltd, Cambridge
2. Baldisserotto C, Popovich C, Giovanardi M
et al (2016) Photosynthetic aspects and lipid
profiles in the mixotrophic alga Neochloris
oleoabundans as useful parameters for biodiesel
production. Algal Res 16:255–265
3. Gray CG, Lasiter AD, Leblond JD (2009)
Mono- and digalactosyldiacylglycerol composition of dinoflagellates. III. Four cold-adapted,
peridinin-containing taxa and the presence of
trigalactosyldiacylglycerol as an additional glycolipid. Eur J Phycol 44:439–445
4. Da Costa E, Silva J, Mendonc ¸a SH, Abreu MH
and Domingues MR (2016) Lipidomic
approaches towards deciphering glycolipids from
microalgae as a reservoir of bioactive lipids. Mar
Drugs.
Doi:
https://doi.org/10.3390/
md14050101
5. Yao L, Gerde JA, Lee SL, Wang T, Harrata KA
(2015) Microalgae lipid characterization. J Agric
Food Chem 63:1773–1787
6. Nygren H, Sepp€ anen-Laakso T, Castillo S et al
(2011) Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies
of body fluids and tissues. Methods Mol Biol
708:247–257
7. Zhao YY, Wu SP, Liu S et al (2014) Ultraperformance liquid chromatography–mass spectrometry as a sensitive and powerful technology
in lipidomic applications. Chem Biol Interact
220:181–192
8. Cutignano A, Luongo E, Nuzzo G et al (2016)
Profiling of complex lipids in marine microalgae by UHPLC/tandem mass spectrometry.
Algal Res 17:348–358
9. Pluskal T, Castillo S, Villar-Briones A, Ores ˇic ˇ M
(2010) MZmine 2: modular framework for
processing, visualizing, and analyzing mass
spectrometry-based molecular profile data.
BMC Bioinformatics 11:395
10. Katajamaa M, Miettinen J, Ores ˇic ˇ M (2006)
MZmine: toolbox for processing and visualization of mass spectrometry based molecular profile data. Bioinformatics 22:634–636
222
Heli Nygren et al.
the standard compounds are used for validation of retention
times.
Acknowledgments
The authors thank Ulla Lahtinen and Airi Hyrk€ as for excellent
technical assistance in preparing extracts of algal samples.
References
1. Kumari P, Kumar M, Reddy CRK et al (2013)
Algal lipids, fatty acids and sterols. In: Dominguez H (ed) Functional ingredients from algae
for foods and nutraceuticals. Woodhead Publishing Ltd, Cambridge
2. Baldisserotto C, Popovich C, Giovanardi M
et al (2016) Photosynthetic aspects and lipid
profiles in the mixotrophic alga Neochloris
oleoabundans as useful parameters for biodiesel
production. Algal Res 16:255–265
3. Gray CG, Lasiter AD, Leblond JD (2009)
Mono- and digalactosyldiacylglycerol composition of dinoflagellates. III. Four cold-adapted,
peridinin-containing taxa and the presence of
trigalactosyldiacylglycerol as an additional glycolipid. Eur J Phycol 44:439–445
4. Da Costa E, Silva J, Mendonc ¸a SH, Abreu MH
and Domingues MR (2016) Lipidomic
approaches towards deciphering glycolipids from
microalgae as a reservoir of bioactive lipids. Mar
Drugs.
Doi:
https://doi.org/10.3390/
md14050101
5. Yao L, Gerde JA, Lee SL, Wang T, Harrata KA
(2015) Microalgae lipid characterization. J Agric
Food Chem 63:1773–1787
6. Nygren H, Sepp€ anen-Laakso T, Castillo S et al
(2011) Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies
of body fluids and tissues. Methods Mol Biol
708:247–257
7. Zhao YY, Wu SP, Liu S et al (2014) Ultraperformance liquid chromatography–mass spectrometry as a sensitive and powerful technology
in lipidomic applications. Chem Biol Interact
220:181–192
8. Cutignano A, Luongo E, Nuzzo G et al (2016)
Profiling of complex lipids in marine microalgae by UHPLC/tandem mass spectrometry.
Algal Res 17:348–358
9. Pluskal T, Castillo S, Villar-Briones A, Ores ˇic ˇ M
(2010) MZmine 2: modular framework for
processing, visualizing, and analyzing mass
spectrometry-based molecular profile data.
BMC Bioinformatics 11:395
10. Katajamaa M, Miettinen J, Ores ˇic ˇ M (2006)
MZmine: toolbox for processing and visualization of mass spectrometry based molecular profile data. Bioinformatics 22:634–636
222
Heli Nygren et al.