the species comprising the 41 remaining clones have been previously reported to
form sclerotia.
2.3.2.2 Identification of Isolates by the Culture Method
The number and classification of isolates obtained from sclerotia collected from soil
from Mt. Ontake cultured on CMA (pH 6.2), CMA (pH 5.0), and MMN (pH 5.8) are
shown in Fig. 2.3 The majority (34) of the 38 isolates obtained were ascomycetes.
CMA adjusted to pH 5.0 yielded the greatest number of isolates, likely due to the fact
that the soil from which the sclerotia were collected was acidic (pH 4.86). The most
commonly isolated species was Oidiodendron pilicola (Ascomycetes:
Myxotrichaceae). A total of seven O. pilicola isolates were obtained using CMA
adjusted to pH 5.0 and MMN adjusted to pH 5.8. Oidiodendron spp. have previously
been isolated in large numbers from acidic soil (Bååth et al. 1984) but have not been
reported to form sclerotia. Given that acidobacteria, which are found in low-pH
Fig. 2.3 Number and classification of isolates obtained from sclerotia collected from soil from
Mt. Ontake (Gifu Prefecture). CMA cornmeal agar, MMN modified Melin–Norkrans medium with
0.5% glucose content (Nonoyama et al. 2016)
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K. Narisawa et al.