Acknowledgements This work was conducted in the framework of the PIREN-Seine research
programme (www.piren-seine.fr), a component of the Zone Atelier Seine within the international
Long Term Socio Ecological Research (LTSER) network.
References
1. Billen G, Garnier J, Mouchel JM et al (2007) The Seine system: introduction to a
multidisciplinary approach of the functioning of a regional river system. Sci Total Environ
375:1–12. https://doi.org/10.1016/j.scitotenv.2006.12.001
2. Chapman PM (2007) Determining when contamination is pollution – weight of evidence de
terminations for sediments and effluents. Environ Int 33:492–501
3. European Commission (EC) (2000) Directive 2000/60/EC of the European Parliament and of
the Council of 23 October 2000 establishing a framework for Community action in the field of
water policy. Off J Eur Union 327:1–73
4. Kabir ER, Rahman MS, Rahman I (2015) A review on endocrine disruptors and their possible
impacts on human health. Environ Toxicol Pharmacol 40:241–258
5. Wagner M, Kienle C, Vermeirssen ELM et al (2017) Endocrine disruption and in vitro eco
toxicology: recent advances and approaches. Adv Biochem Eng Biotechnol 157:1–58
6. Teil MJ, Tlili K, Blanchard M et al (2014) Polychlorinated biphenyls, polybrominated diphenyl
ethers, and phthalates in roach from the Seine River basin (France): impact of densely urbanized
areas. Arch Environ Contam Toxicol 66:41–57
7. Sumpter J, Jobling S (1995) Vitellogenesis as a biomarker for estrogenic contamination of the
aquatic environment. Environ Health Perspect 103:173–178
8. Tousova Z, Oswald P, Slobodnik J et al (2017) European demonstration program on the effectbased and chemical identification and monitoring of organic pollutants in European surface
waters. Sci Total Environ 601–602:1849–1868
9. Leusch FDL, Neale PA, Hebert A et al (2017) Analysis of the sensitivity of in vitro bioassays
for androgenic, progestagenic, glucocorticoid, thyroid and estrogenic activity: suitability for
drinking and environmental waters. Environ Int 99:120–130
10. Wangmo C, Jarque S, Hilscherová K et al (2018) In vitro assessment of sex steroids and related
compounds in water and sediments – a critical review. Environ Sci Process Impacts 20:270–287
11. Jugan ML, Oziol L, Bimbot M et al (2009) In vitro assessment of thyroid and estrogenic
endocrine disruptors in wastewater treatment plants, rivers and drinking water supplies in the
greater Paris area (France). Sci Total Environ 407:3579–3587
12. Gasperi J, Moreau-Guigon E, Labadie P et al (2011) Contamination de la Seine par les
micropolluants organiques: Effet des conditions hydriques et de l’urbanisation. PIREN-Seine,
Phase V, Rapport de synthèse 2007–2010
13. Miège C, Karolak S, Gabet V et al (2009) Evaluation of estrogenic disrupting potency in aquatic
environments and urban wastewaters by combining chemical and biological analysis. Trends
Anal Chem 28:186–195
14. EFSA (2015) Scientific opinion on the risks to public health related to the presence of bisphenol
A (BPA) in foodstuffs: opinion on BPA. EFSA J 13:3978
15. ANSES (2017) Élaboration de VTR par voie orale fondée sur des effets reprotoxiques pour les
nonylphénols. Saisine n
2017-SA-0211
16. Jarošová B, Bláha L, Giesy JP et al (2014) What level of estrogenic activity determined by
in vitro assays in municipal waste waters can be considered as safe? Environ Int 64:98–109
17. Fenet H, Gomez E, Pillon A et al (2003) Estrogenic activity in water and sediments of a French
river: contribution of alkylphenols. Arch Environ Contam Toxicol 44:1–6
Experience Gained from Ecotoxicological Studies in the Seine River and. . .
265
programme (www.piren-seine.fr), a component of the Zone Atelier Seine within the international
Long Term Socio Ecological Research (LTSER) network.
References
1. Billen G, Garnier J, Mouchel JM et al (2007) The Seine system: introduction to a
multidisciplinary approach of the functioning of a regional river system. Sci Total Environ
375:1–12. https://doi.org/10.1016/j.scitotenv.2006.12.001
2. Chapman PM (2007) Determining when contamination is pollution – weight of evidence de
terminations for sediments and effluents. Environ Int 33:492–501
3. European Commission (EC) (2000) Directive 2000/60/EC of the European Parliament and of
the Council of 23 October 2000 establishing a framework for Community action in the field of
water policy. Off J Eur Union 327:1–73
4. Kabir ER, Rahman MS, Rahman I (2015) A review on endocrine disruptors and their possible
impacts on human health. Environ Toxicol Pharmacol 40:241–258
5. Wagner M, Kienle C, Vermeirssen ELM et al (2017) Endocrine disruption and in vitro eco
toxicology: recent advances and approaches. Adv Biochem Eng Biotechnol 157:1–58
6. Teil MJ, Tlili K, Blanchard M et al (2014) Polychlorinated biphenyls, polybrominated diphenyl
ethers, and phthalates in roach from the Seine River basin (France): impact of densely urbanized
areas. Arch Environ Contam Toxicol 66:41–57
7. Sumpter J, Jobling S (1995) Vitellogenesis as a biomarker for estrogenic contamination of the
aquatic environment. Environ Health Perspect 103:173–178
8. Tousova Z, Oswald P, Slobodnik J et al (2017) European demonstration program on the effectbased and chemical identification and monitoring of organic pollutants in European surface
waters. Sci Total Environ 601–602:1849–1868
9. Leusch FDL, Neale PA, Hebert A et al (2017) Analysis of the sensitivity of in vitro bioassays
for androgenic, progestagenic, glucocorticoid, thyroid and estrogenic activity: suitability for
drinking and environmental waters. Environ Int 99:120–130
10. Wangmo C, Jarque S, Hilscherová K et al (2018) In vitro assessment of sex steroids and related
compounds in water and sediments – a critical review. Environ Sci Process Impacts 20:270–287
11. Jugan ML, Oziol L, Bimbot M et al (2009) In vitro assessment of thyroid and estrogenic
endocrine disruptors in wastewater treatment plants, rivers and drinking water supplies in the
greater Paris area (France). Sci Total Environ 407:3579–3587
12. Gasperi J, Moreau-Guigon E, Labadie P et al (2011) Contamination de la Seine par les
micropolluants organiques: Effet des conditions hydriques et de l’urbanisation. PIREN-Seine,
Phase V, Rapport de synthèse 2007–2010
13. Miège C, Karolak S, Gabet V et al (2009) Evaluation of estrogenic disrupting potency in aquatic
environments and urban wastewaters by combining chemical and biological analysis. Trends
Anal Chem 28:186–195
14. EFSA (2015) Scientific opinion on the risks to public health related to the presence of bisphenol
A (BPA) in foodstuffs: opinion on BPA. EFSA J 13:3978
15. ANSES (2017) Élaboration de VTR par voie orale fondée sur des effets reprotoxiques pour les
nonylphénols. Saisine n
2017-SA-0211
16. Jarošová B, Bláha L, Giesy JP et al (2014) What level of estrogenic activity determined by
in vitro assays in municipal waste waters can be considered as safe? Environ Int 64:98–109
17. Fenet H, Gomez E, Pillon A et al (2003) Estrogenic activity in water and sediments of a French
river: contribution of alkylphenols. Arch Environ Contam Toxicol 44:1–6
Experience Gained from Ecotoxicological Studies in the Seine River and. . .
265