Experience Gained from Ecotoxicological
Studies in the Seine River and Its Drainage
Basin Over the Last Decade: Applicative
Examples and Research Perspectives
M. Bonnard, I. Barjhoux, O. Dedourge-Geffard, A. Goutte, L. Oziol,
M. Palos-Ladeiro, and A. Geffard
Contents
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 244
2 Value of In Vitro Bioassays for an Ex Situ Evaluation of Water Contamination: The Case
of Endocrine Disruptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 245
2.1 In Vitro Endocrine Disruptor Bioassays in Water Contamination Monitoring . . . . . . 246
2.2 Comparison of In Vitro Bioassays to Chemical Analysis in Water Contamination
Monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 246
2.3 In Vitro Bioassays Applied to Sediment Matrix in Aquatic Environment Quality
Monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 248
2.4 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 250
3 Biomarkers for the In Situ Biomonitoring of Water Contamination . . . . . . . . . . . . . . . . . . . . . . . 250
3.1 Presentation of Sentinel Species and Their Advantages in Ecotoxicological Studies 250
3.2 Monitoring of Oxidative Stress in Fish Models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 252
3.3 Value of Digestive Activities as Biomarkers in Two Aquatic Invertebrates:
Application to the Seine River Basin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 253
3.4 Value of Haemocytes of Zebra Mussels for the Development of Immune
and Genotoxic Biomarkers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
4 Combining Multidisciplinary Data for a Global Environmental Quality Diagnosis:
Development of an Integrated Approach . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
4.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
4.2 The WOE Methodology: Towards an Index of Ecological Disturbance . . . . . . . . . . . . 260
The copyright year of the original version of this chapter was corrected from 2019 to 2020.
A correction to this chapter can be found at https://doi.org/10.1007/698_2020_667
M. Bonnard (*), I. Barjhoux, O. Dedourge-Geffard, M. Palos-Ladeiro, and A. Geffard
Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO, Reims, France
e-mail: marc.bonnard@univ-reims.fr
A. Goutte
PSL University, UMR 7619 METIS, SU/CNRS/EPHE, Paris, France
L. Oziol
Université Paris-Sud, CNRS, AgroParisTech, UMR 8079 ESE, Orsay, France
Nicolas Flipo, Pierre Labadie, and Laurence Lestel (eds.), The Seine River Basin,
Hdb Env Chem (2021) 90: 243–268, https://doi.org/10.1007/698_2019_384,
© The Author(s) 2020, corrected publication 2020, Published online: 3 June 2020
243
Studies in the Seine River and Its Drainage
Basin Over the Last Decade: Applicative
Examples and Research Perspectives
M. Bonnard, I. Barjhoux, O. Dedourge-Geffard, A. Goutte, L. Oziol,
M. Palos-Ladeiro, and A. Geffard
Contents
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 244
2 Value of In Vitro Bioassays for an Ex Situ Evaluation of Water Contamination: The Case
of Endocrine Disruptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 245
2.1 In Vitro Endocrine Disruptor Bioassays in Water Contamination Monitoring . . . . . . 246
2.2 Comparison of In Vitro Bioassays to Chemical Analysis in Water Contamination
Monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 246
2.3 In Vitro Bioassays Applied to Sediment Matrix in Aquatic Environment Quality
Monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 248
2.4 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 250
3 Biomarkers for the In Situ Biomonitoring of Water Contamination . . . . . . . . . . . . . . . . . . . . . . . 250
3.1 Presentation of Sentinel Species and Their Advantages in Ecotoxicological Studies 250
3.2 Monitoring of Oxidative Stress in Fish Models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 252
3.3 Value of Digestive Activities as Biomarkers in Two Aquatic Invertebrates:
Application to the Seine River Basin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 253
3.4 Value of Haemocytes of Zebra Mussels for the Development of Immune
and Genotoxic Biomarkers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
4 Combining Multidisciplinary Data for a Global Environmental Quality Diagnosis:
Development of an Integrated Approach . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
4.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
4.2 The WOE Methodology: Towards an Index of Ecological Disturbance . . . . . . . . . . . . 260
The copyright year of the original version of this chapter was corrected from 2019 to 2020.
A correction to this chapter can be found at https://doi.org/10.1007/698_2020_667
M. Bonnard (*), I. Barjhoux, O. Dedourge-Geffard, M. Palos-Ladeiro, and A. Geffard
Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO, Reims, France
e-mail: marc.bonnard@univ-reims.fr
A. Goutte
PSL University, UMR 7619 METIS, SU/CNRS/EPHE, Paris, France
L. Oziol
Université Paris-Sud, CNRS, AgroParisTech, UMR 8079 ESE, Orsay, France
Nicolas Flipo, Pierre Labadie, and Laurence Lestel (eds.), The Seine River Basin,
Hdb Env Chem (2021) 90: 243–268, https://doi.org/10.1007/698_2019_384,
© The Author(s) 2020, corrected publication 2020, Published online: 3 June 2020
243