Alnus
glutinosa
Frozen at
À20
C
and stored until
use. Following the
method described
by Bundschuh
et al. (2011b)
Acclimation food:
preconditioned
leaves
Experiment food:
conditioning medium
(Dang et al. 2005)
inoculated with
leaves previously
conditioned directly
in the river
10 days
After being cut in
leaf discs of 2.0 cm
in diameter, but
before being dried at
60
C for 24 h and
weighed
Soaked for 24 h in
tap water before
being used in the
experiment
Acclimation
food:
ad libitum
Feeding, accumulation and
growth study
Zubrod
et al. (2010)
Alnus
glutinosa
Frozen at
À20
C
and stored until
use. Following the
method described
by Zubrod et al.
(2010)
Acclimation food:
preconditioned
leaves
Experiment food:
conditioning medium
(Dang et al. 2005)
inoculated with
leaves previously
conditioned directly
in the river
10 days
After being cut in
leaf discs of 2.0 cm
in diameter, but
before being dried at
60
C for 24 h and
weighed. Soaked for
48 h in tap water
before being used in
the experiment
Acclimation
food:
ad libitum
Feeding an survival study
Zubrod
et al. (2014)
Alnus
glutinosa
Frozen at
À20
C
and stored until use
Conditioned in
medium with leaves
that were previously
conditioned directly
in the river for
14 days
12 days
Acclimation
food:
ad libitum
Toxicity and
feeding study
Zubrod
et al. (2015)
Alnus
glutinosa
Frozen at
À20
C
and stored until use
Conditioned in
stream water with
leaves that were previously conditioned
directly in the river
for 14 days and for
14 days in the lab
13 days
Feeding behaviour and physiological
responses
Zubrod
et al. (2017)
Alnus
spp.
Air-dried and
stored
River water with
detritus
At least
10 days
After being air-dried
Ad libitum
Laboratory
breeding
program
Bloor
(2010)
(continued)
Feeding Behavioural Studies with Freshwater Gammarus spp.: The. . .
19