Alnus
glutinosa
Frozen at
À20
C
and stored until
use. Following the
method described
by Bundschuh
et al. (2009)
Acclimation food:
preconditioned
leaves
Experiment food:
conditioning medium
(Dang et al. 2005)
inoculated with
leaves previously
conditioned directly
in the river
12 days
After being cut in
leaf discs of 1.6 cm
in diameter and dried
Feeding preferences study
Bundschuh
et al.
(2011a)
Alnus
glutinosa
Froze at
À20
C
until use
Acclimation food:
preconditioned alder
leaves
Experiment food:
conditioned in a
nutrient medium with
added leaves that
were previously conditioned directly in
the river
10 days
After being cut in
leaf discs of 2 cm in
diameter, but before
been dried at 60
C
for 24 h
Preconditioned
alder leaves fed
ad libitum
Feeding study
Bundschuh
et al.
(2011b)
Alnus
glutinosa
Froze at
À20
C
until use
Acclimation food:
preconditioned alder
leaves
Experiment food:
conditioned in a
nutrient medium with
added leaves that
were previously conditioned directly in
the river
10 days
After being cut in
leaf discs of 2 cm in
diameter, but before
been dried at 60
C
for 24 h
Feeding study
Bundschuh
et al. (2013)
(continued)
Feeding Behavioural Studies with Freshwater Gammarus spp.: The. . .
15