8
Figure 25: adding the spirulina culture to the new medium (LMK) ....................................... 37
Figure 26: Spirulina culture after seeding ............................................................................... 38
Figure 27: 10 L of LMK medium prepared ............................................................................ 38
Figure 28: the seeded culture inside the aquarium.................................................................. 38
Figure 29: the collected spirulina culture ............................................................................... 39
Figure 30: centrifugation ........................................................................................................ 39
Figure 31: the tubes after centrifugation ................................................................................. 39
Figure 32: an oven set at 100°C .............................................................................................. 39
Figure 33: vortex mixing of the dried pellet and water .......................................................... 39
Figure 34: the mixture after vortex ......................................................................................... 39
Figure 35: the addition of HCl 2N to the suspension ............................................................. 40
Figure 36: water bath set at 80°C ........................................................................................... 40
Figure 37: chloroform ............................................................................................................. 40
Figure 38: the result of adding chloroform to the supernatant ............................................... 40
Figure 39: stirring water bath .................................................................................................. 40
Figure 40: addition of chloroform .......................................................................................... 41
Figure 41: an oven set at 40°C ................................................................................................ 41
Figure 42: results after drying ................................................................................................. 41
Figure 43: placing a sample on a microscope slide ................................................................ 41
Figure 44: placing the sample under the microscope .............................................................. 41
Figure 45: the concentrated spirulina culture ......................................................................... 42
Figure 46: microcentrifuge tubes filled with spirulina ........................................................... 42
Figure 47: the microcentrifuge ............................................................................................... 43
Figure 48: the tubes after centrifugation ................................................................................. 43
Figure 49: spirulina biomass in a crystallizer ......................................................................... 44
Figure 50: the spirulina biomass ............................................................................................. 44