4
3.4 Systematic ................................................................................................................. 25
3.5 Morphological features ............................................................................................. 26
3.6 Habitat ....................................................................................................................... 26
3.7 Applications of spirulina ........................................................................................... 27
3.8 Cultivation ................................................................................................................. 27
3.9 Growing Conditions .................................................................................................. 28
3.9.1 Light ................................................................................................................... 28
3.9.2 Temperature ....................................................................................................... 28
3.9.3 pH ....................................................................................................................... 28
3.10
Growing Technique ............................................................................................... 28
3.10.1 Seeding ............................................................................................................... 29
3.10.2 Agitation ............................................................................................................ 29
3.10.3 Shading .............................................................................................................. 29
3.10.4 Harvesting .......................................................................................................... 29
3.10.5 Extrusion and Drying ......................................................................................... 30
3.10.6 Culture mediums ................................................................................................ 30
CHAPTER II: MATERIALS AND METHODS
1 Cultivation
1.1 Materials .................................................................................................................... 34
1.2 Methods ..................................................................................................................... 34
2 Extraction of Polyhydroxybutyrate (PHB)
2.1 Materials .................................................................................................................... 38
2.2 Method ...................................................................................................................... 39
3 Harvesting and drying
3.1 Materials .................................................................................................................... 41
3.2 Method ...................................................................................................................... 42