119
7.4.2 Pure Culture Studies
Results from pure cultures of hydrocarbon-degrading bacteria are equivocal with
respect to the influence of pressure on growth and hydrocarbon degradation potential. Elevated partial pressure of methane and oxygen, but not total pressure, is
inhibitory to Methylococcus capsulatus (Bath), isolated from sediment and water,
and Methylomicrobium album BG8, isolated from freshwater (Soni et al. 1998).
ƉƌĞƐƐƵƌŝnjĂƟŽŶ
ǁŝƚŚ E Ϯ Žƌ , ϰ
ŽƉƟĐĂů ŽŶůŝŶĞ
ŵŽŶŝƚŽƌŝŶŐ ŽĨ K Ϯ
ĐŽŶĐĞŶƚƌĂƟŽŶ
;WƌĞ^ĞŶƐͿ
ĚŝīĞƌĞŶƚ
ĞŶǀŝƌŽŶŵĞŶƚĂů
ĐŽŶĚŝƟŽŶ ĂƉƉůŝĐĂďůĞ
;Ğ͘Ő͘ƚĞŵƉĞƌĂƚƵƌĞͿ
UHDFWRUYROXPH
PO
SUHVVXUH
PD[03D
± 1 WR SUHVVXUL]H
PD[ 03D
± &+ WR SUHVVXUL]H
PD[ 03D
± 2 RQOLQHPHDVXUHPHQW
Ϳ
Ϳ
Fig. 7.3 High hydrostatic pressure characteristic of the deep sea can be simulated within these
reactors allowing the examination of biological processes under deep sea conditions. (a) The reactors made from stainless steel and bronze can withstand maximum pressure of 400 bar. Reactors
are equipped with oxygen sensors to continuously monitor oxygen and/or (b) NIR optical system
to simultaneously monitor biodegradation of hydrocarbon gases
7 Biodegradation of Petroleum Hydrocarbons in the Deep Sea
7.4.2 Pure Culture Studies
Results from pure cultures of hydrocarbon-degrading bacteria are equivocal with
respect to the influence of pressure on growth and hydrocarbon degradation potential. Elevated partial pressure of methane and oxygen, but not total pressure, is
inhibitory to Methylococcus capsulatus (Bath), isolated from sediment and water,
and Methylomicrobium album BG8, isolated from freshwater (Soni et al. 1998).
ƉƌĞƐƐƵƌŝnjĂƟŽŶ
ǁŝƚŚ E Ϯ Žƌ , ϰ
ŽƉƟĐĂů ŽŶůŝŶĞ
ŵŽŶŝƚŽƌŝŶŐ ŽĨ K Ϯ
ĐŽŶĐĞŶƚƌĂƟŽŶ
;WƌĞ^ĞŶƐͿ
ĚŝīĞƌĞŶƚ
ĞŶǀŝƌŽŶŵĞŶƚĂů
ĐŽŶĚŝƟŽŶ ĂƉƉůŝĐĂďůĞ
;Ğ͘Ő͘ƚĞŵƉĞƌĂƚƵƌĞͿ
UHDFWRUYROXPH
PO
SUHVVXUH
PD[03D
± 1 WR SUHVVXUL]H
PD[ 03D
± &+ WR SUHVVXUL]H
PD[ 03D
± 2 RQOLQHPHDVXUHPHQW
Ϳ
Ϳ
Fig. 7.3 High hydrostatic pressure characteristic of the deep sea can be simulated within these
reactors allowing the examination of biological processes under deep sea conditions. (a) The reactors made from stainless steel and bronze can withstand maximum pressure of 400 bar. Reactors
are equipped with oxygen sensors to continuously monitor oxygen and/or (b) NIR optical system
to simultaneously monitor biodegradation of hydrocarbon gases
7 Biodegradation of Petroleum Hydrocarbons in the Deep Sea