2.3 Research on High-Efficient Anaerobic Fermentation Technology …
43
Fig. 2.22 Changes of cumulative biogas production and biological gas production in different
stages of CHMP-AF of straws
(2) Response relationship of identified proteins and methane production
Production of methane from microorganisms is a process capable of reducing methyl
in compounds containing one carbon to methane under joint actions of many coenzymes and enzymes in one-carbon metabolism. This was realized by the cooperation
of plenty of archaea proteins and small quantities of bacterial proteins. This study
revealed that during SHPT, three pathways, i.e. acetate, CO 2 reduction, and methylotrophic pathways, separately involving 10, 6, and one protein for methanogenesis
were active. In addition, it can be seen that the proteins in the acetate pathway held
the vast majority of the total activity. Observably, expression of acetyl-CoA decarbonylase/synthase complex (sub-units alpha, beta, delta, epsilon, and gamma) was
found at higher levels in SHPT, especially in Stage II, during which acetic acid was
used as the solo carbon source and energy to catalyze the decarboxylation of acetylCoA as it was decomposed to CO 2 . Therefore, it is a critical enzyme for the pathway
through which methanogenesis uses acetate.
Other proteins mainly involved in the acetate pathway included: (i) sub-units A
and H of tetrahydromethanopterin S-methyltransferase; (ii) phosphate acetyltransferase; (iii) acetate kinase; and (iv) acetyl-CoA synthetase. Remarkably, based on
the metaproteomic analysis, these enzymes all belonged to Euryarchaeota and to
Methanosarcina, and Methanosaeta. It was found in Stages II and III that acetate
43
Fig. 2.22 Changes of cumulative biogas production and biological gas production in different
stages of CHMP-AF of straws
(2) Response relationship of identified proteins and methane production
Production of methane from microorganisms is a process capable of reducing methyl
in compounds containing one carbon to methane under joint actions of many coenzymes and enzymes in one-carbon metabolism. This was realized by the cooperation
of plenty of archaea proteins and small quantities of bacterial proteins. This study
revealed that during SHPT, three pathways, i.e. acetate, CO 2 reduction, and methylotrophic pathways, separately involving 10, 6, and one protein for methanogenesis
were active. In addition, it can be seen that the proteins in the acetate pathway held
the vast majority of the total activity. Observably, expression of acetyl-CoA decarbonylase/synthase complex (sub-units alpha, beta, delta, epsilon, and gamma) was
found at higher levels in SHPT, especially in Stage II, during which acetic acid was
used as the solo carbon source and energy to catalyze the decarboxylation of acetylCoA as it was decomposed to CO 2 . Therefore, it is a critical enzyme for the pathway
through which methanogenesis uses acetate.
Other proteins mainly involved in the acetate pathway included: (i) sub-units A
and H of tetrahydromethanopterin S-methyltransferase; (ii) phosphate acetyltransferase; (iii) acetate kinase; and (iv) acetyl-CoA synthetase. Remarkably, based on
the metaproteomic analysis, these enzymes all belonged to Euryarchaeota and to
Methanosarcina, and Methanosaeta. It was found in Stages II and III that acetate